Artículos de revistas
Extraction Of Recombinant Cytochrome B5 From Disrupted Escherichia Coli Cells With An Aqueous Two-phase System In A Continuous Perforated Rotating Disc Contactor
Registro en:
Biotechnology Techniques. , v. 11, n. 9, p. 641 - 643, 1997.
0951208X
2-s2.0-0030874790
Autor
Porto A.L.F.
Lima-Filho J.L.
Aires-Barros M.R.
Cabral J.M.S.
Tambourgi E.B.
Institución
Resumen
A continuous perforated rotating disc contactor was used to extract recombinant cytochrome b5 from disrupted Escherichia coli cells with an aqueous two-phase system. About 4.9 mg (44%) total protein and 5 mg (75%) cytochrome b5 were recovered to the salt-rich phase, using PEG (18% w/w)-KH(2PO4/K2HPO4) (16% w/w) salt system. The dispersed phase (PEG) hold up increased with increasing rotor velocity. The mass transfer coefficient and separation efficiency for cytochrome, normalized for the PEG hold up, depended upon the disc's rotation speed. 11 9 641 643 Bradford, M.M., (1976) Anal. Biochem., 72, pp. 248-254 Carneiro-da-Cunha, M.G., Aires-Barros, M.R., Tarabourgi, E.B., Cabral, J.M.S., (1994) Biotechnol. Tech., 8, pp. 413-418 Jafarabad, K.R., Sawant, S.B., Joshi, J.B., Sidkar, S.K., (1992) Chem. Eng. Sci., 47, pp. 57-68 Kumar, A., Hartland, S., (1982) Trans. Instn. Chem. Engrs., 60, pp. 35-39 Mathews, F.S., Czerwinski, E.W., Cytochrome b5 and cytochrome b5 reductase from a chemical and x-ray diffraction viewpoint (1989) The Enzymes of Biological Membranes, 4, pp. 235-300. , A.N. Martonosi, ed. New York : Plenum Press Raghav Rao, K.S.M., Szlag, D.C., Sidkar, S.K., (1991) Chem. Eng. J., 46, pp. B75-B81 Reman, G.H., Van De Vusse, J.G., (1955) Genie Chimique, 74, pp. 106-111 Sarmento, M.J., Pires, M.J., Cabrai, J.M.S., Aires-Barros, M.R., (1994) J. Chromat., 688, pp. 117-120 Sawant, S.B., Sidkar, S.B., Joshi, J.B., (1990) Biotechnol. Bioeng., 36, pp. 109-115 Schmidt, A.S., Andrews, B.A., Ansejo, J.A., (1996) Biotechnol. Bioeng., 35, pp. 617-626 Tambourgi, E.B., Pereira, J.A.F.R., Perazoli, L.A., (1993) Latin American Applied Research, 23, pp. 257-260 Venâncio, A., Teixeira, J.A., (1995) Bioprocess Eng., 13, pp. 251-255 Von Bodman, S.B., Schuller, M.A., Jollie, D.R., Slingar, S.G., (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 9443-9447 Walter, H., Brooks, D.E., Fisher, D., (1985) Partitioning in Aqueous Two-phase Systems, , Academic Press, New York