Extraction Of Recombinant Cytochrome B5 From Disrupted Escherichia Coli Cells With An Aqueous Two-phase System In A Continuous Perforated Rotating Disc Contactor

dc.creatorPorto A.L.F.
dc.creatorLima-Filho J.L.
dc.creatorAires-Barros M.R.
dc.creatorCabral J.M.S.
dc.creatorTambourgi E.B.
dc.date1997
dc.date2015-06-30T14:50:16Z
dc.date2015-11-26T15:07:35Z
dc.date2015-06-30T14:50:16Z
dc.date2015-11-26T15:07:35Z
dc.date.accessioned2018-03-28T22:18:00Z
dc.date.available2018-03-28T22:18:00Z
dc.identifier
dc.identifierBiotechnology Techniques. , v. 11, n. 9, p. 641 - 643, 1997.
dc.identifier0951208X
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0030874790&partnerID=40&md5=3af7b5c6c578f2de694f086e050a4d35
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/100295
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/100295
dc.identifier2-s2.0-0030874790
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1257493
dc.descriptionA continuous perforated rotating disc contactor was used to extract recombinant cytochrome b5 from disrupted Escherichia coli cells with an aqueous two-phase system. About 4.9 mg (44%) total protein and 5 mg (75%) cytochrome b5 were recovered to the salt-rich phase, using PEG (18% w/w)-KH(2PO4/K2HPO4) (16% w/w) salt system. The dispersed phase (PEG) hold up increased with increasing rotor velocity. The mass transfer coefficient and separation efficiency for cytochrome, normalized for the PEG hold up, depended upon the disc's rotation speed.
dc.description11
dc.description9
dc.description641
dc.description643
dc.descriptionBradford, M.M., (1976) Anal. Biochem., 72, pp. 248-254
dc.descriptionCarneiro-da-Cunha, M.G., Aires-Barros, M.R., Tarabourgi, E.B., Cabral, J.M.S., (1994) Biotechnol. Tech., 8, pp. 413-418
dc.descriptionJafarabad, K.R., Sawant, S.B., Joshi, J.B., Sidkar, S.K., (1992) Chem. Eng. Sci., 47, pp. 57-68
dc.descriptionKumar, A., Hartland, S., (1982) Trans. Instn. Chem. Engrs., 60, pp. 35-39
dc.descriptionMathews, F.S., Czerwinski, E.W., Cytochrome b5 and cytochrome b5 reductase from a chemical and x-ray diffraction viewpoint (1989) The Enzymes of Biological Membranes, 4, pp. 235-300. , A.N. Martonosi, ed. New York : Plenum Press
dc.descriptionRaghav Rao, K.S.M., Szlag, D.C., Sidkar, S.K., (1991) Chem. Eng. J., 46, pp. B75-B81
dc.descriptionReman, G.H., Van De Vusse, J.G., (1955) Genie Chimique, 74, pp. 106-111
dc.descriptionSarmento, M.J., Pires, M.J., Cabrai, J.M.S., Aires-Barros, M.R., (1994) J. Chromat., 688, pp. 117-120
dc.descriptionSawant, S.B., Sidkar, S.B., Joshi, J.B., (1990) Biotechnol. Bioeng., 36, pp. 109-115
dc.descriptionSchmidt, A.S., Andrews, B.A., Ansejo, J.A., (1996) Biotechnol. Bioeng., 35, pp. 617-626
dc.descriptionTambourgi, E.B., Pereira, J.A.F.R., Perazoli, L.A., (1993) Latin American Applied Research, 23, pp. 257-260
dc.descriptionVenâncio, A., Teixeira, J.A., (1995) Bioprocess Eng., 13, pp. 251-255
dc.descriptionVon Bodman, S.B., Schuller, M.A., Jollie, D.R., Slingar, S.G., (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 9443-9447
dc.descriptionWalter, H., Brooks, D.E., Fisher, D., (1985) Partitioning in Aqueous Two-phase Systems, , Academic Press, New York
dc.languageen
dc.publisher
dc.relationBiotechnology Techniques
dc.rightsfechado
dc.sourceScopus
dc.titleExtraction Of Recombinant Cytochrome B5 From Disrupted Escherichia Coli Cells With An Aqueous Two-phase System In A Continuous Perforated Rotating Disc Contactor
dc.typeArtículos de revistas


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