Artículos de revistas
Modulatory Effects Of Yerba Maté (ilex Paraguariensis) On The Pi3k-akt Signaling Pathway
Registro en:
Molecular Nutrition And Food Research. , v. 57, n. 10, p. 1882 - 1885, 2013.
16134125
10.1002/mnfr.201200834
2-s2.0-84884913712
Autor
Arcari D.P.
Santos J.C.
Gambero A.
Ferraz L.F.C.
Ribeiro M.L.
Institución
Resumen
The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, glucose blood level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, glucose blood levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 57 10 1882 1885 Ahima, R.S., Flier, J.S., Adipose tissue as an endocrine organ (2000) Trends Endocrinol. Metab., 11, pp. 327-332 Ahima, R.S., Digging deeper into obesity (2011) J. Clin. Invest., 121, pp. 2076-2079 Fruhbeck, G., Gomez-Ambrosi, J., Muruzabal, F.J., Burrell, M.A., The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation (2001) Am. J. Physiol. Endocrinol. 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