Artículos de revistas
Identification Of Coagulase-negative Staphylococci From Bovine Intramammary Infection By Matrix-assisted Laser Desorption Ionization-time Of Flight Mass Spectrometry
Registro en:
Journal Of Clinical Microbiology. American Society For Microbiology, v. 52, n. 5, p. 1658 - 1663, 2014.
951137
10.1128/JCM.03032-13
2-s2.0-84899507921
Autor
Tomazi T.
Goncalves J.L.
Barreiro J.R.
De Campos Braga P.A.
Prada E Silva L.F.
Eberlin M.N.
Dos Santos M.V.
Institución
Resumen
Coagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). 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