dc.creatorTomazi T.
dc.creatorGoncalves J.L.
dc.creatorBarreiro J.R.
dc.creatorDe Campos Braga P.A.
dc.creatorPrada E Silva L.F.
dc.creatorEberlin M.N.
dc.creatorDos Santos M.V.
dc.date2014
dc.date2015-06-25T18:01:20Z
dc.date2015-11-26T15:03:07Z
dc.date2015-06-25T18:01:20Z
dc.date2015-11-26T15:03:07Z
dc.date.accessioned2018-03-28T22:13:58Z
dc.date.available2018-03-28T22:13:58Z
dc.identifier
dc.identifierJournal Of Clinical Microbiology. American Society For Microbiology, v. 52, n. 5, p. 1658 - 1663, 2014.
dc.identifier951137
dc.identifier10.1128/JCM.03032-13
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84899507921&partnerID=40&md5=ce7fb6b1eff4ae41eea4bbe291454d42
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/87552
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/87552
dc.identifier2-s2.0-84899507921
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1256536
dc.descriptionCoagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n=108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n=80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
dc.description52
dc.description5
dc.description1658
dc.description1663
dc.descriptionWatts, J.L., Etiological agents of bovine mastitis (1988) Vet. Microbial., 16, pp. 41-66. , http://dx.doi.org/10.1016/0378-1135(88)90126-5
dc.descriptionBaird-Parker, A.C., A classification of micrococci and staphylococci based on physiological and biochemical tests (1963) J. Gen. Microbiol., 30, pp. 409-427. , http://dx.doi.org/10.1099/00221287-30-3-409
dc.descriptionDevriese, L.A., De Keyser, H., Prevalence of different species of coagulasenegative staphylococci on teats and in milk samples from dairy cows (1980) J. Dairy Res., 47, pp. 155-158. , http://dx.doi.org/10.1017/S0022029900020999
dc.descriptionDevriese, L.A., Schleifer, K.H., Adegoke, G.O., Identification of coagulasenegative staphylococci from farm animals (1985) J. Appl. Bacteriol., 58, pp. 45-55. , http://dx.doi.org/10.1111/j.1365-2672.1985.tb01428.x
dc.descriptionKloos, W.E., Schleifer, K.H., Simplified scheme for routine identification of human Staphylococcus species (1975) J. Clin. Microbiol., 1, pp. 82-88
dc.descriptionSchleifer, K.H., Kilpper-Balz, R., Devriese, L.A., Staphylococcus arletta sp nov., S equorum sp nov., and S kloosii sp nov.: Three new coagulasenegative, novobiocin-resistant species from animals (1984) Syst. Appl. Microbiol., 5, pp. 501-509. , http://dx.doi.org/10.1016/S0723-2020(84)80007-7
dc.descriptionWatts, J.L., Nickerson, S.C., A comparison of the STAPH-Ident and STAPH-Trac systems to conventional methods in the identification of staphylococci isolated from bovine udders (1986) Vet. Microbiol., 12, pp. 179-187. , http://dx.doi.org/10.1016/0378-1135(86)90079-9
dc.descriptionPyörälä, S., Taponen, S., Coagulase-negative staphylococci-emerging mastitis pathogens (2009) Vet. Microbiol., 134, pp. 3-8. , http://dx.doi.org/10.1016/j.vetmic.2008.09.015
dc.descriptionCapurro, A., Artursson, K., Waller, K.P., Bengtsson, B., Ericsson-Unnerstad, H., Aspán, A., Comparison of a commercialized phenotyping system, antimicrobial susceptibility testing, and tuf gene sequence-based genotyping for species-level identification of coagulase-negative staphylococci isolated from cases of bovine mastitis (2009) Vet. Microbiol., 134, pp. 327-333. , http://dx.doi.org/10.1016/j.vetmic.2008.08.028
dc.descriptionSupré, K., Haesebrouck, F., Zadoks, R.N., Vaneechoutte, M., Piepers, S., De Vliegher, S., Some coagulase-negative Staphylococcus species affect udder health more than others (2011) J. Dairy Sci., 94, pp. 2329-2340. , http://dx.doi.org/10.3168/jds.2010-3741
dc.descriptionBirgersson, A., Jonsson, P., Holmberg, O., Species identification and some characteristics of coagulase-negative staphylococci isolated from bovine udders (1992) Vet. Microbiol., 31, pp. 181-189. , http://dx.doi.org/10.1016/0378-1135(92)90076-6
dc.descriptionMatthews, K.R., Harmon, R.J., Langlois, B.E., Prevalence of Staphylococcus species during the periparturient period in primiparous and multiparous cows (1992) J. Dairy Sci., 75, pp. 1835-1839. , http://dx.doi.org/10.3168/jds.S0022-0302(92)77942-9
dc.descriptionPiessens, V., Van Coillie, E., Verbist, B., Supré, K., Braem, G., Van Nuffel, A., De Vuyst, L., De Vliegher, S., Distribution of coagulasenegative Staphylococcus species from milk and environment of dairy cows differs between herds (2011) J. Dairy Sci., 94, pp. 2933-2944. , http://dx.doi.org/10.3168/jds.2010-3956
dc.descriptionThorberg, B.M., Danielsson-Tham, M.L., Emanuelson, U., Persson Waller, K., Bovine subclinical mastitis caused by different types of coagulasenegative staphylococci (2009) J. Dairy Sci., 92, pp. 4962-4970. , http://dx.doi.org/10.3168/jds.2009-2184
dc.descriptionSantos, O.C., Barros, E.M., Brito, M.A., Bastos Mdo, C., Dos Santos, K.R.N., Giambiagi-Demarval, M., Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene (2008) Vet. Microbiol., 130, pp. 134-140. , http://dx.doi.org/10.1016/j.vetmic.2007.12.009
dc.descriptionBes, M., Guerin-Faublee, V., Meugnier, H., Etienne, J., Freney, J., Improvement of the identification of staphylococci isolated from bovine mammary infections using molecular methods (2000) Vet. Microbiol., 71, pp. 287-294. , http://dx.doi.org/10.1016/S0378-1135(99)00163-7
dc.descriptionThorberg, B.M., Brandstrom, B., Evaluation of two commercial systems and a new identification scheme based on solid substrates for identifying coagulase-negative staphylococci from bovine mastitis (2000) J. Vet. Med. B Infect. Dis. Vet. Public Health, 47, pp. 683-691. , http://dx.doi.org/10.1046/j.1439-0450.2000.00399.x
dc.descriptionBritten, A.M., The role of diagnostic microbiology in mastitis control programs (2012) Vet. Clin. North Am. Food Anim. Pract., 28, pp. 187-202. , http://dx.doi.org/10.1016/j.cvfa.2012.03.006
dc.descriptionBenagli, C., Rossi, V., Dolina, M., Tonolla, M., Petrini, O., Matrixassisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria (2011) PLoS One, 6, pp. e16424. , http://dx.doi.org/10.1371/journal.pone.0016424
dc.descriptionBizzini, A., Greub, G., Matrix-assisted laser desorption ionization-time of flight mass spectrometry, a revolution in clinical microbial identification (2010) Clin. Microbiol. Infect., 16, pp. 1614-1619. , http://dx.doi.org/10.1111/j.1469-0691.2010.03311.x
dc.descriptionCarbonnelle, E., Beretti, J.L., Cottyn, S., Quesne, G., Berche, P., Nassif, X., Ferroni, A., Rapid identification of staphylococci isolated in clinical microbiology laboratories by matrix-assisted laser desorption ionization-time of flight mass spectrometry (2007) J. Clin. Microbiol., 45, pp. 2156-2161. , http://dx.doi.org/10.1128/JCM.02405-06
dc.descriptionMatsuda, N., Matsuda, M., Notake, S., Yokokawa, H., Kawamura, Y., Hiramatsu, K., Kikuchi, K., Evaluation of a simple protein extraction method for species identification of clinically relevant staphylococci by matrix-assisted laser desorption ionization-time of flight mass spectrometry (2012) J. Clin. Microbiol., 50, pp. 3862-3866. , http://dx.doi.org/10.1128/JCM.01512-12
dc.descriptionSpanu, T., De Carolis, E., Fiori, B., Sanguinetti, M., D'Inzeo, T., Fadda, G., Posteraro, B., Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry in comparison to rpoB gene sequencing for species identification of bloodstream infection staphylococcal isolates (2011) Clin. Microbiol. Infec., 17, pp. 44-49. , http://dx.doi.org/10.1111/j.1469-0691.2010.03181.x
dc.descriptionBarreiro, J.R., Ferreira, C.R., Sanvido, G.B., Kostrzewa, M., Maier, T., Wegemann, B., Böttcher, V., Dos Santos, M.V., Short communication: Identification of subclinical cow mastitis pathogens in milk by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2010) J. Dairy Sci., 93, pp. 5661-5667. , http://dx.doi.org/10.3168/jds.2010-3614
dc.description(2004) Microbiological Procedures for the Diagnosis of Bovine Udder Infection and Determination of Milk Quality, , National Mastitis Council, 4th ed. National Mastitis Council, Madison, WI
dc.descriptionSambrook, J., Russell, D.W., (2001) Molecular Cloning: A Laboratory Manual, , 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
dc.descriptionGoh, S.H., Potter, S., Wood, J.O., Hemmingsen, S.M., Reynolds, R.P., Chow, A.W., HSP60 gene sequences as universal targets for microbial species identification: Studies with coagulase-negative staphylococci (1996) J. Clin. Microbiol., 34, pp. 818-823
dc.descriptionRyzhov, V., Fenselau, C., Characterization of the protein subset desorbed by MALDI from whole bacterial cells (2001) Anal. Chem., 73, pp. 746-750. , http://dx.doi.org/10.1021/ac0008791
dc.descriptionRuegg, P.L., New perspectives in udder health management (2012) Vet. Clin. North Am. Food Anim. Pract., 28, pp. 149-163. , http://dx.doi.org/10.1016/j.cvfa.2012.03.001
dc.descriptionBannerman, T.L., Staphylococcus, Micrococcus, and other catalasepositive cocci that grow aerobically (2003) Manual of Clinical Microbiology, pp. 84-404. , Murray PR, Barron EJ, Pfaller MA, Tenover FC, Yolken RH ed, 10th ed. ASM Press, Washington, DC
dc.descriptionMacFaddin, J.F., (1997) Biochemical Tests for Identification of Medical Bacteria., , Williams & Wilkins, Baltimore, MD
dc.descriptionBarros, E.M., Iorio, N.L.P., Bastos Mdo, C., Dos Santos, K.R.N., Giambiagide Marval, M., Species-level identification of clinical staphylococcal isolates based on polymerase chain reaction-restriction fragment length polymorphism analysis of a partial groEL gene sequence (2007) Diagn. Microbiol. Infect. Dis., 59, pp. 251-257. , http://dx.doi.org/10.1016/j.diagmicrobio.2007.05.004
dc.descriptionGoh, S.H., Santucci, Z., Kloos, W.E., Faltyn, M., George, C.G., Driedger, D., Hemmingsen, S.M., Identification of Staphylococcus species and subspecies by the chaperonin 60 gene identification method and reverse checkerboard hybridization (1997) J. Clin. Microb., 35, pp. 3116-3121
dc.descriptionPoyart, C., Quesne, G., Boumaila, C., Trieu-Cuot, P., Rapid and accurate species level identification of coagulase-negative staphylococci by using the sodA gene as a target (2001) J. Clin. Microbiol., 39, pp. 4296-4301. , http://dx.doi.org/10.1128/JCM.39.12.4296-4301.2001
dc.descriptionHeikens, E., Fleer, A., Paauw, A., Florijn, A., Fluit, A.C., Comparison of genotypic and phenotypic methods for species-level identification of clinical isolates of coagulase-negative staphylococci (2005) J. Clin. Microbiol., 43, pp. 2286-2290. , http://dx.doi.org/10.1128/JCM.43.5.2286-2290.2005
dc.descriptionLoonen, A.J., Jansz, A.R., Bergland, J.N., Valkenburg, M., Wolffs, P.F., Van Den Brule, A.J., Comparative study using phenotypic, genotypic, and proteomics methods for identification of coagulase-negative staphylococci (2012) J. Clin. Microbiol., 50, pp. 1437-1439. , http://dx.doi.org/10.1128/JCM.06746-11
dc.descriptionMaier, T., Kostrzewa, M., Fast and reliable MALDI-TOF MS-based microorganism identification (2007) Chim. Oggi., 25, pp. 68-71
dc.descriptionBraga, P.A.C., Tata, A., Santos, V.G., Barreiro, J.R., Schwab, N.V., Santos, M.V., Eberlin, M.N., Ferreira, C.R., Bacterial identification: From agar plate to the mass spectrometer (2013) RSC Adv., 3, pp. 994-1008. , http://dx.doi.org/10.1039/c2ra22063f
dc.descriptionDe Vliegher, S., Laevens, H., Devriese, L.A., Opsomer, G., Leroy, J.L., Barkema, H.W., Kruif, A., Prepartum teat apex colonization with Staphylococcus chromogenes in dairy heifers is associated with low somatic cell count in early lactation (2003) Vet. Microbiol., 92, pp. 245-252. , http://dx.doi.org/10.1016/S0378-1135(02)00363-2
dc.descriptionDufour, S., Dohoo, I.R., Barkema, H.W., Des Côteaux, L., De Vries, T.J., Reyher, K.K., Roy, J.P., Scholl, D.T., Epidemiology of coagulase-negative staphylococci intramammary infection in dairy cattle and the effect of bacteriological culture misclassification (2012) J. Dairy Sci., 95, pp. 3110-3124. , http://dx.doi.org/10.3168/jds.2011-5164
dc.descriptionPiessens, V., De Vliegher, S., Verbist, B., Braem, G., Van Nuffel, A., De Vuyst, L., Heyndrickx, M., Van Coillie, E., Intra-species diversity and epidemiology varies among coagulase-negative Staphylococcus species causing bovine intramammary infections (2012) Vet. Microbiol., 155, pp. 62-71. , http://dx.doi.org/10.1016/j.vetmic.2011.08.005
dc.descriptionRajala-Schultz, P.J., Smith, K.L., Hogan, J.S., Love, B.C., Antimicrobial susceptibility of mastitis pathogens from first lactation and older cows (2004) Vet. Microbiol., 102, pp. 33-42. , http://dx.doi.org/10.1016/j.vetmic.2004.04.010
dc.descriptionTaponen, S., Simojoki, H., Haveri, M., Larsen, H.D., Pyörälä, S., Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP (2006) Vet. Microbiol., 115, pp. 199-207. , http://dx.doi.org/10.1016/j.vetmic.2006.02.001
dc.descriptionPate, M., Zdovc, I., Avberšek, J., Ocepek, M., Pengov, A., Podpečan, O., Coagulase-negative staphylococci from non-mastitic bovine mammary gland: Characterization of Staphylococcus chromogenes and Staphylococcus haemolyticus by antibiotic susceptibility testing and pulsed-field gel electrophoresis (2012) J. Dairy Res., 79, pp. 129-134. , http://dx.doi.org/10.1017/S0022029911000811
dc.descriptionTaponen, S., Koort, J., Björkroth, J., Saloniemi, H., Pyörälä, S., Bovine intramammary infections caused by coagulase-negative staphylococci may persist throughout lactation according to amplified fragment length polymorphism-based analysis (2007) J. Dairy Sci., 90, pp. 3301-3307. , http://dx.doi.org/10.3168/jds.2006-860
dc.descriptionQuirk, T., Fox, L.K., Hancock, D.D., Capper, J., Wenz, J., Park, J., Intramammary infections and teat canal colonization with coagulasenegative staphylococci after postmilking teat disinfection: Species-specific responses (2012) J. Dairy Sci., 95, pp. 1906-1912. , http://dx.doi.org/10.3168/jds.2011-4898
dc.descriptionFox, L.K., Besser, T.E., Jackson, S.M., Evaluation of a coagulase-negative variant of Staphylococcus aureus as a cause of intramammary infections in a herd of dairy cattle (1996) J. Am. Vet. Med. Assoc., 209, pp. 1143-1146
dc.descriptionPark, J.Y., Fox, L.K., Seo, K.S., McGuire, M.A., Park, Y.H., Rurangirwa, F.R., Sischo, W.M., Bohach, G.A., Detection of classical and newly described staphylococcal superantigen genes in coagulase-negative staphylococci isolated from bovine intramammary infections (2011) Vet. Microbiol., 147, pp. 149-154. , http://dx.doi.org/10.1016/j.vetmic.2010.06.021
dc.languageen
dc.publisherAmerican Society for Microbiology
dc.relationJournal of Clinical Microbiology
dc.rightsaberto
dc.sourceScopus
dc.titleIdentification Of Coagulase-negative Staphylococci From Bovine Intramammary Infection By Matrix-assisted Laser Desorption Ionization-time Of Flight Mass Spectrometry
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución