Identification Of Coagulase-negative Staphylococci From Bovine Intramammary Infection By Matrix-assisted Laser Desorption Ionization-time Of Flight Mass Spectrometry

dc.creatorTomazi T.
dc.creatorGoncalves J.L.
dc.creatorBarreiro J.R.
dc.creatorDe Campos Braga P.A.
dc.creatorPrada E Silva L.F.
dc.creatorEberlin M.N.
dc.creatorDos Santos M.V.
dc.date2014
dc.date2015-06-25T18:01:20Z
dc.date2015-11-26T15:03:07Z
dc.date2015-06-25T18:01:20Z
dc.date2015-11-26T15:03:07Z
dc.date.accessioned2018-03-28T22:13:58Z
dc.date.available2018-03-28T22:13:58Z
dc.identifier
dc.identifierJournal Of Clinical Microbiology. American Society For Microbiology, v. 52, n. 5, p. 1658 - 1663, 2014.
dc.identifier951137
dc.identifier10.1128/JCM.03032-13
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84899507921&partnerID=40&md5=ce7fb6b1eff4ae41eea4bbe291454d42
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/87552
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/87552
dc.identifier2-s2.0-84899507921
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1256536
dc.descriptionCoagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n=108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n=80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
dc.description52
dc.description5
dc.description1658
dc.description1663
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dc.languageen
dc.publisherAmerican Society for Microbiology
dc.relationJournal of Clinical Microbiology
dc.rightsaberto
dc.sourceScopus
dc.titleIdentification Of Coagulase-negative Staphylococci From Bovine Intramammary Infection By Matrix-assisted Laser Desorption Ionization-time Of Flight Mass Spectrometry
dc.typeArtículos de revistas


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