Artículos de revistas
Production, Purification And Application Of Extracellular Chitinase From Cellulosimicrobium Cellulans 191 [produção, Purificação E Aplicação Da Quitinase Extracelular De Cellulosimicrobium Cellulans 191]
Registro en:
Brazilian Journal Of Microbiology. , v. 40, n. 3, p. 623 - 630, 2009.
15178382
2-s2.0-70349507282
Autor
Fleuri L.F.
Kawaguti H.Y.
Sato H.H.
Institución
Resumen
This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25°C and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25°C and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts. 40 3 623 630 Balasubramanian, N., Juliet, A.A., Srikalavani, P., Lalithakumari, D., Release and regeneration of protoplasts from Trichothecium roseum (2003) Can. J. 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