Production, Purification And Application Of Extracellular Chitinase From Cellulosimicrobium Cellulans 191 [produção, Purificação E Aplicação Da Quitinase Extracelular De Cellulosimicrobium Cellulans 191]

dc.creatorFleuri L.F.
dc.creatorKawaguti H.Y.
dc.creatorSato H.H.
dc.date2009
dc.date2015-06-26T13:38:13Z
dc.date2015-11-26T14:59:50Z
dc.date2015-06-26T13:38:13Z
dc.date2015-11-26T14:59:50Z
dc.date.accessioned2018-03-28T22:11:19Z
dc.date.available2018-03-28T22:11:19Z
dc.identifier
dc.identifierBrazilian Journal Of Microbiology. , v. 40, n. 3, p. 623 - 630, 2009.
dc.identifier15178382
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-70349507282&partnerID=40&md5=0f9ced562d8095a446870099889310bc
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/92982
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/92982
dc.identifier2-s2.0-70349507282
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1256108
dc.descriptionThis study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25°C and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25°C and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.
dc.description40
dc.description3
dc.description623
dc.description630
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dc.languagept
dc.publisher
dc.relationBrazilian Journal of Microbiology
dc.rightsaberto
dc.sourceScopus
dc.titleProduction, Purification And Application Of Extracellular Chitinase From Cellulosimicrobium Cellulans 191 [produção, Purificação E Aplicação Da Quitinase Extracelular De Cellulosimicrobium Cellulans 191]
dc.typeArtículos de revistas


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