Artículos de revistas
The Rus Operon Genes Are Differentially Regulated When Acidithiobacillus Ferrooxidans Lr Is Kept In Contact With Metal Sulfides
Registro en:
Current Microbiology. , v. 57, n. 4, p. 375 - 380, 2008.
3438651
10.1007/s00284-008-9208-7
2-s2.0-50249093201
Autor
Carlos C.
Reis F.C.
Vicentini R.
Madureira D.J.
Ottoboni L.M.M.
Institución
Resumen
Acidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy from the oxidation of ferrous iron or reduced sulfur compounds. In this bacterium, the proteins encoded by the rus operon are involved in electron transfer from Fe(II) to O2, and the first two proteins in this pathway also participate in the electron transfer pathway from Fe(II) to NAD(P). In this work we analyzed the expression, by real-time PCR, of the eight genes from the rus operon when A. ferrooxidans LR was grown in the presence of iron (control) and then kept in contact with chalcopyrite (CuFeS2) and covellite (CuS). A small decrease in rus operon gene expression was observed in the presence of chalcopyrite, while in the presence of covellite the expression of these genes showed a remarkable decrease. These results can be explained by the absence of ferrous iron in covellite. To explain the expression difference observed between the gene cyc1 and the gene rus, we investigated the information content presented at the Translation Initiation Site (TIS) of both genes. cyc1 showed a highly information content (8.4 bits) that can maximize translation, and rus showed a less favorable context (5.5 bits). Our hypothesis is that the energetic metabolism in A. ferrooxidans may be controlled at the transcriptional and posttranscriptional level by different mechanisms. © 2008 Springer Science+Business Media, LLC. 57 4 375 380 Appia-Ayme, C., Guiliani, N., Ratouchniak, J., Bonnefoy, V., Characterization of an operon encoding two c-type cytochromes, an aa 3-type cytochrome oxidase and rusticyanin in Thiobacillus ferrooxidans ATCC 33020 (1999) Appl Environ Microbiol, 65, pp. 4781-4787 Bengrine, A., Guiliani, N., Appia-Ayme, C., Jedlicki, E., Holmes, D.S., Chippaux, M., Bonnefoy, V., Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC 33020 strain (1998) Biochim Biophys Acta, 1443, pp. 99-112 Brasseur, G., Levican, G., Bonnefoy, V., Holmes, D., Jedlicki, E., Lemesle-Meunier, D., Apparent redundancy of electron transfer pathways via bc(1) complexes and terminal oxidases in the extremophilic chemolithoautotrophic Acidithiobacillus ferrooxidans (2004) Biochim Biophys Acta, 1656, pp. 114-126 Bruscella, P., Appia-Ayme, C., Levicán, G., Ratouchniak, J., Jedlicki, E., Holmes, D.S., Bonnefoy, V., Differential expression of two bc 1 complexes in the strict acidophilic chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans suggests a model for their respective roles in iron or sulfur oxidation (2007) Microbiology, 153, pp. 102-110 Elbehti, A., Brasseur, G., Lemesle-Meunier, D., First evidence for existence of an uphill electron transfer through the bc 1 and NADH-Q oxidoreductase complexes of the acidophilic obligate chemolithotrophic ferrous iron-oxidizing bacterium Thiobacillus ferrooxidans (2000) J Bacteriol, 182, pp. 3602-3606 Garcia Jr., O., Isolation and purification of Thiobacillus ferrooxidans and Thiobacillus thiooxidans from some coal and uranium mines of Brazil (1991) Revista Microbiol, 22, pp. 1-6 Hauryliuk, V., Ehrenberg, M., Two-step selection of mRNAs in initiation of protein synthesis (2006) Mol Cell, 22, pp. 155-156 Holmes, D.S., Bonnefoy, V., Rawlings, D.E., Johnson, B.D., Genetic and bioinformatics insights into iron and sulfur oxidation mechanisms of bioleaching organisms (2007) Biomining, pp. 281-307. , Springer-Verlag New York Ingledew, W.J., T. ferrooxidans, the bioenergetics of an acidophilic chemolithotrophic bacteria (1982) Biochim Biophys Acta, 683, pp. 89-117 Livak, K.J., Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC(T) method (2001) Methods, 25, pp. 402-408 Quatrini, R., Appia-Ayme, C., Denis, Y., Insights into the iron and sulfur energetic metabolism of Acidithiobacillus ferrooxidans by microarray transcriptome profiling (2006) Hydrometallurgy, 83, pp. 263-272 Ramirez, P., Guiliani, N., Valenzuela, L., Beard, S., Jerez, C.A., Differential protein expression during growth of Acidithiobacillus ferrooxidans on ferrous iron, sulfur compounds, or metal sulfides (2004) Appl Environ Microbiol, 70, pp. 4491-4498 Rawlings, D.E., Heavy metal mining using microbes (2002) Annu Rev Microbiol, 56, pp. 65-91 Steitz, J.A., Jakes, K., How ribosomes select initiator regions in mRNA: Base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli (1975) Proc Natl Acad Sci USA, 72, pp. 4734-4738 Tuovinen, O.H., Kelly, D.P., Biology of Thiobacillus ferrooxidans in relation to the microbiological leaching of sulphide ore (1972) Z Allg Mikrobiol, 12, pp. 311-346 Vicentini, R., Menossi, M., TISs-ST: A web server to evaluate polymorphic translation initiation sites and their reflections on the secretory targets (2007) BMC Bioinform, 8, p. 160 Winderickx, J., Castro, J.M., (1994) Pratical Course in Molecular Biology of Microorganisms, p. 59. , Universidade Federal de Ouro Preto-MG, January 23-February 11 Yarzábal, A., Brasseur, G., Ratouchniak, J., Lund, K., Lemesle-Meunier, D., Demoss, J.A., Bonnefoy, V., The high molecular weight cytochrome c Cyc2 of Acidithiobacillus ferrooxidans is an outer membrane protein (2002) J Bacteriol, 184, pp. 313-317 Yarzábal, A., Appia-Ayme, C., Ratouchniak, J., Bonnefoy, V., Regulation of the expression of the Acidithiobacillus ferrooxidans rus operon encoding two cytochromes c, a cytochrome oxidase and rusticyanin (2004) Microbiology, 150, pp. 2113-2123