The Rus Operon Genes Are Differentially Regulated When Acidithiobacillus Ferrooxidans Lr Is Kept In Contact With Metal Sulfides

dc.creatorCarlos C.
dc.creatorReis F.C.
dc.creatorVicentini R.
dc.creatorMadureira D.J.
dc.creatorOttoboni L.M.M.
dc.date2008
dc.date2015-06-30T19:31:02Z
dc.date2015-11-26T14:45:02Z
dc.date2015-06-30T19:31:02Z
dc.date2015-11-26T14:45:02Z
dc.date.accessioned2018-03-28T21:54:08Z
dc.date.available2018-03-28T21:54:08Z
dc.identifier
dc.identifierCurrent Microbiology. , v. 57, n. 4, p. 375 - 380, 2008.
dc.identifier3438651
dc.identifier10.1007/s00284-008-9208-7
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-50249093201&partnerID=40&md5=9639dd436d21f92bd903f99fb842534c
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/106569
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/106569
dc.identifier2-s2.0-50249093201
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1252351
dc.descriptionAcidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy from the oxidation of ferrous iron or reduced sulfur compounds. In this bacterium, the proteins encoded by the rus operon are involved in electron transfer from Fe(II) to O2, and the first two proteins in this pathway also participate in the electron transfer pathway from Fe(II) to NAD(P). In this work we analyzed the expression, by real-time PCR, of the eight genes from the rus operon when A. ferrooxidans LR was grown in the presence of iron (control) and then kept in contact with chalcopyrite (CuFeS2) and covellite (CuS). A small decrease in rus operon gene expression was observed in the presence of chalcopyrite, while in the presence of covellite the expression of these genes showed a remarkable decrease. These results can be explained by the absence of ferrous iron in covellite. To explain the expression difference observed between the gene cyc1 and the gene rus, we investigated the information content presented at the Translation Initiation Site (TIS) of both genes. cyc1 showed a highly information content (8.4 bits) that can maximize translation, and rus showed a less favorable context (5.5 bits). Our hypothesis is that the energetic metabolism in A. ferrooxidans may be controlled at the transcriptional and posttranscriptional level by different mechanisms. © 2008 Springer Science+Business Media, LLC.
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dc.description375
dc.description380
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dc.languageen
dc.publisher
dc.relationCurrent Microbiology
dc.rightsfechado
dc.sourceScopus
dc.titleThe Rus Operon Genes Are Differentially Regulated When Acidithiobacillus Ferrooxidans Lr Is Kept In Contact With Metal Sulfides
dc.typeArtículos de revistas


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