Artículos de revistas
Influence Of The Fermentation Parameters And Optimisation Of Isomaltulose Production From Free Erwinia Sp. D12 Cells Using Response Surface Methodology
Registro en:
Process Biochemistry. , v. 42, n. 3, p. 472 - 479, 2007.
13595113
10.1016/j.procbio.2006.09.013
2-s2.0-33847396891
Autor
Kawaguti H.Y.
Sato H.H.
Institución
Resumen
The food industry is constantly seeking novel ingredients to improve existing products or to allow for the introduction of new products. Isomaltulose is a reducing sugar with a sweet taste and very similar physical and organoleptic properties to those of sucrose. The strain Erwinia sp. is able to convert sucrose into isomaltulose. A two level rotatory central composite design and response surface methodology were applied to verify the influence and conditions for the production of isomaltulose by Erwinia sp. D12 free-cells in a batch process. The statistical analysis carried out at a confidence level of 90% gave a coefficient of determination of 0.90, and the polynomial model resulted in a response surface and contour curve that indicated the best parameters for the conversion of sucrose into isomaltulose as follows: temperature 35 °C, pH 6.5, wet cell mass 10% and sucrose 35%. The free-cells of Erwinia sp. D12 were recycled during repeated-batch processes to produce isomaltulose from sucrose obtaining high isomaltulose yields. © 2006 Elsevier Ltd. All rights reserved. 42 3 472 479 Huang, J.H., Hsu, L.H., Su, Y.C., Conversion of sucrose to isomaltulose by Klebsiella planticola CCRC 19112 (1998) J Ind Microbiol Biotechnol, 21, pp. 22-27 Takazoe, I., Frostell, G., Ohta, K., Topitsoglou, V., Sasaki, N., Palatinose-a sucrose substitute (1985) Swed Dent J, 9, pp. 81-87 Krastanov, A., Yoshida, A., Production of palatinose using Serratia plymuthica cells immobilized in chitosan (2003) J Ind Microbiol Biotechnol, 30, pp. 593-598 Lina, B.A.R., Smits-van prooije, A.E., Waalkens-berendsen, D.H., Embryotoxicity/teratogenicity study with isomaltulose (palatinose) in rats (1997) Food Chem Toxicol, 35, pp. 309-314 Lina, B.A.R., Jonker, D., Kozianowski, G., Isomaltulose (Isomaltulose®): a review of biological and toxicological studies (2002) Food Chem Toxicol, 40, pp. 1375-1381 Weidenhagen, R., Lorenz, S., Isomaltulose [6(α-glucopyranoside)-fructofuranose], ein neues bakterielles umwandlungsprodukt der saccharose (1957) Z Zuckerindustrie, 7, pp. 533-534 Cheetham, P.S.J., Garrett, C., Clark, J., Isomaltulose production using immobilized cells (1984) Biotechnol Bioeng, 27, pp. 471-481 Mcallister, M., Kelly, C.T., Doyle, E., Fogarty, W.M., The isomaltulose synthesing enzyme of Serratia plymuthica (1990) Biotechnol Lett, 12, pp. 667-672 Wu, L., Birch, R.G., Characterization of Pantoeae dispersa UQ68J: producer of a highly efficient sucrose isomerase for isomaltulose biosynthesis (2004) J Appl Microbiol, 97, pp. 93-103 Kawaguti, H.Y., Manrich, E., Fleuri, L.F., Sato, H.H., Production of glucosyltransferase by Erwinia sp. using experimental design and response surface methodology (2005) Braz J Microbiol, 36, pp. 227-234 Kawaguti, H.Y., Buzzato, M.F., Orsi, D.C., Suzuki, G.T., Sato, H.H., Effect of the additives polyethylenimine and glutaraldehyde on the immobilization of Erwinia sp D12 cells in calcium alginate for isomaltulose production (2006) Process Biochems, 41, pp. 2035-2040 Tsuyuki, K., Sugitani, Y., Miyata, Y., Ebashi, T., Nakajima, Y., Isolation and characterization of trehalulose-producing bacteria from Thailand soil (1992) J Gen Appl Microbiol, 38, pp. 483-490 Véronèse, T., Perlot, P., Mechanism of sucrose conversion by the sucrose isomerase of Serratia plymuthica ATCC 15928 (1999) Enzyme Microb Technol, 24, pp. 263-269 Moraes, A.L.L., Steckelberg, C., Sato, H.H., Pinheiro, A., Produção de isomaltulose a partir da transformação enzimática da sacarose, utilizando-se Erwinia sp. D12 imobilizada com alginato de cálcio (2005) Ciênc Tecnol Aliment, 25, pp. 95-102 Li, X., Zhao, C., Na, Q., Zhang, D., Substrate induction isomaltulose synthase in a newly isolated Klebsiella sp. LX 3 (2003) J Appl Microbiol, 95, pp. 2131-2136