Artículos de revistas
Urinalysis: Comparison Between Microscopic And Flow Cytometry Analysis [urinálise: Comparação Entre Microscopia óptica E Citometria De Fluxo]
Registro en:
Jornal Brasileiro De Patologia E Medicina Laboratorial. , v. 42, n. 3, p. 157 - 162, 2006.
16762444
2-s2.0-33747444980
Autor
Bottini P.V.
Garlipp C.R.
Institución
Resumen
Introduction: Urinalysis is a high demand procedure, with large amount of manual labor and poorly standardized. The purpose of this investigation was to analyze the performance of an automated system based on flow cytometry for routine urinalysis. Material and methods: We analyzed 1,140 urine samples by light field microscopy and by flow cytometry (UF-100/SYSMEX). For the precision study of the UF-100, we calculated the within-run and between-run coefficients of variation using two different levels of commercial controls and four different urine samples. Results: Erythrocytes and leukocytes counts by the two methods showed an agreement of 91% and 93%, respectively. Casts, epithelial cells and bacteria counts by the UF-100 showed a significant overlap when compared to microscopic analysis. Intra assay precision (within-run) ranged from 4% to 155% and interassay precision (between-run) varied from 3% to 25%, depending on the considered parameter. Conclusion: Flow cytometry is a precise and reproducible technique, with a strong correlation with the results obtained by microscopic analysis. Flow cytometry allows a better workflow and a new manner of reporting and interpreting routine urinalysis. 42 3 157 162 Andriolo, A., Avaliação de um sistema totalmente automatizado para a realização do exame de urina de rotina: Urina tipo I (1999) NewsLab, 36, pp. 76-80 Ben-Ezra, J., Bork, L., Macpherson, R.A., Evaluation of the Sysmex UF-100 automated urinalysis analyzer (1998) Clin Chem, 44, pp. 92-95 Delanghe, J.R., The role of automated urine particle flow cytometry in clinical practice (2000) Clin Chim Acta, 301, pp. 1-18 Dimech, W., Roney, K., Evaluation of an automated urinalysis system for testing urine chemistry, microscopy and culture (2002) Pathology, 34, pp. 170-177 European urinalysis guidelines (2000) Scand J Clin Lab Invest, 60, p. 231 Fenili, D., Pirovano, B., The automation of sediment urinalysis using a new urine flow cytometer (UF-100) (1998) Clin Chem Lab Med, 36 (12), pp. 909-917 Keijzer, M.H., Brandts, R.W., Flow cytometry and the urine laboratory: Field evaluation of the SYSMEX UF-100. SYSMEX (1997) J Int, 7, pp. 117-122 Kim, Y., Quantitative analysis of urine sediment using newly designed centrifuge tubes (2002) Ann Clin Lab Sci, 32, pp. 55-60 Kouri, T.T., Evaluation of SYSMEX UF-100 urine flow cytometer vs. chamber counting of supravitally stained specimens and conventional bacterial cultures (1999) Am J Clin Pathol, 112, pp. 25-35 Langlois, M.R., Automated flow cytometry compared with an automated dipstick reader for urinalysis (1999) Clin Chem, 45, pp. 118-122 (1995) Urinalysis and Collection, Transpor Tation and Preservation of Urine Specimens Approved Guideline: NCCLS Document GP 16-A, , Wayne, PA: NCCLS Ottiger, C., Huber, A.R., Quantitative urine particle analysis: Integrative approach for the optimal combination of automation with UF-100 and microscopic review with KOVA cell chamber (2003) Clin Chem, 49, pp. 617-623 Winkel, P., Statland, B.E., Jorgensen, K., Urine microscopy, an ill defined method, examined by a multifactorial technique (1974) Clin Chem, 20, pp. 436-439