Artículos de revistas
Identification Of Novel Candidate Genes For Globin Regulation In Erythroid Cells Containing Large Deletions Of The Human β-globin Gene Cluster
Registro en:
Blood Cells, Molecules, And Diseases. , v. 37, n. 2, p. 82 - 90, 2006.
10799796
10.1016/j.bcmd.2006.07.003
2-s2.0-33748785158
Autor
de Andrade T.G.
Peterson K.R.
Cunha A.F.
Moreira L.S.
Fattori A.
Saad S.T.O.
Costa F.F.
Institución
Resumen
The genetic mechanisms underlying the continued expression of the γ-globin genes during the adult stage in deletional hereditary persistence of fetal hemoglobin (HPFH) and δβ-thalassemias are not completely understood. Herein, we investigated the possible involvement of transcription factors, using the suppression subtractive hybridization (SSH) method as an initial screen to identify differentially expressed transcripts in reticulocytes from a normal and a HPFH-2 subject. Some of the detectable transcripts may participate in globin gene regulation. Quantitative real-time PCR (qRT-PCR) experiments confirmed the downregulation of ZHX2, a transcriptional repressor, in two HPFH-2 subjects and in a carrier of the Sicilian δβ-thalassemia trait. The chromatin remodeling factors ARID1B and TSPYL1 had a very similar pattern of expression with an incremental increase in HPFH and decreased expression in δβ-thalassemia. 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