Artículos de revistas
Nongenotoxic Effects And A Reduction Of The Dxr-induced Genotoxic Effects Of Helianthus Annuus Linné (sunflower) Seeds Revealed By Micronucleus Assays In Mouse Bone Marrow
Registration in:
Bmc Complementary And Alternative Medicine. Biomed Central Ltd., v. 14, n. , p. - , 2014.
14726882
10.1186/1472-6882-14-121
2-s2.0-84899491893
Author
Boriollo M.F.G.
Souza L.S.
Resende M.R.
da Silva T.A.
Oliveira N.D.M.S.
Resck M.C.C.
Dias C.T.D.S.
Fiorini J.E.
Institutions
Abstract
Background: This research evaluated the genotoxicity of oil and tincture of H. annuus L. seeds using the micronucleus assay in bone marrow of mice. The interaction between these preparations and the genotoxic effects of doxorubicin (DXR) was also analysed (antigenotoxicity test) .Methods: Experimental groups were evaluated at 24-48 h post treatment with N-Nitroso-N-ethylurea (positive control - NEU), DXR (chemotherapeutic), NaCl (negative control), a sunflower tincture (THALS) and two sources of sunflower oils (POHALS and FOHALS). Antigenotoxic assays were carried out using the sunflower tincture and oils separately and in combination with NUE or DXR. Results: For THALS, analysis of the MNPCEs showed no significant differences between treatment doses (250-2,000 mg.Kg-1) and NaCl. A significant reduction in MNPCE was observed when THALS (2,000 mg.Kg-1) was administered in combination with DXR (5 mg.Kg-1). For POHALS or FOHALS, analysis of the MNPCEs also showed no significant differences between treatment doses (250-2,000 mg.Kg-1) and NaCl. However, the combination DXR + POHALS (2,000 mg.Kg-1) or DXR + FOHALS (2,000 mg.Kg-1) not contributed to the MNPCEs reduction. Conclusions: This research suggests absence of genotoxicity of THALS, dose-, time- and sex-independent, and its combination with DXR can reduce the genotoxic effects of DXR. POHALS and FOHALS also showed absence of genotoxicity, but their association with DXR showed no antigenotoxic effects. © 2014 Boriollo et al.; licensee BioMed Central Ltd. 14
Sunflowers: Helianthus (Compositae-Heliantheae) (1976) Evolution of Crop Plants, pp. 36-38. , London: Longmans Green, Simmonds NW, Heiser CB Earle, F.R., Vanetten, C.H., Clark, T.F., Wolff, I.A., Compositional data on sunflower seed (1968) J Am Oil Chem Soc, 45, pp. 876-879 Salunkhe, D.K., Chavan, J.K., Adsule, R.N., Kadam, S.S., World Oilseeds: Chemistry, Technology and Utilization (1992) Sunflower, , New York: Van Nostrand Reinhold Gonzáles-Pérez, S., Vereijken, J.M., Sunflower proteins: Overview of their physicochemical, structural and functional properties (2007) J Sci Food Agric, 87, pp. 2173-2191 Warner, K.A., Mounts, T.L., List, G.V.R., Effects of added tocopherols on the flavor stability of purified vegetable oils (1990) Inform, 1, p. 326 Ito, T., Tamura, T., Matsumoto, T., Sterol Composition of 19 vegetable oils (1973) J Am Oil Chem Soc, 50, pp. 122-125. , 10.1007/BF02633564, 4707293 Trost, V.W., Characterization of corn oil, soybean oil and sunflower oil nonpolar material (1989) J Am Oil Chem Soc, 66, pp. 325-333 Dupont, H.L., Sullivan, P., Evans, D.G., Vollet, J.J., Ericsson, C.D., Ackerman, P.B., Tjoa, W.S., Prevention of traveler's diarrhea (emporiatric enteritis). Prophylactic administration of subsalicylate bismuth (1980) JAMA, 243, pp. 237-241. , 10.1001/jama.1980.03300290019013, 6985681 Cowan, J.C., Key factors and recent advances in the flavor stability of soybean oil (1966) J Am Oil Chem Soc, 43, pp. 300A-302A. , 318A, 320, 10.1007/BF02682424, 5328054 Bierenbaum, M.L., Fleischman, A.I., Dun, J., Arnold, J., Possible toxic waste factor in coronary heart disease (1975) Lancet, 1, pp. 1008-1010 Purves, D., Harvey, C., Tweats, D., Lumley, C.E., Genotoxity testing: current practices and strategies used by the pharmaceutical industry (1995) Mutagenesis, 10, pp. 297-312. , 10.1093/mutage/10.4.297, 7476265 Varanda, E.A., Atividade mutagênica de plantas medicinais (2006) Rev Ciênc Farm Básica Apl, 27, pp. 1-7 Mateuca, R., Lombaert, N., Aka, P.V., Decordier, I., Kirsch-Volders, M., Chromosomal changes: induction, detection methods and applicability in human biomonitoring (2006) Biochimie, 88, pp. 1515-1531. , 10.1016/j.biochi.2006.07.004, 16919864 Krishna, G., Hayashi, M., In vivo rodent micronucleus assay: protocol, conduct and data interpretation (2000) Mutat Res, 455, pp. 155-166. , 10.1016/S0027-5107(00)00117-2, 11113474 (1997) OECD Guideline for the Testing of Chemicals: Bacterial reverse mutation test, p. 471. , Paris, Organisation for Economic Cooperation and Development (1997) OECD Guideline for the Testing of Chemicals: In vitro mammalian chromosome aberration test, p. 473. , Paris, Organisation for Economic Cooperation and Development (1997) OECD Guideline for the Testing of Chemicals: Mammalian erythrocyte micronucleus test, p. 474. , Paris, Organisation for Economic Cooperation and Development Alves, J.M., Munari, C.C., Neto, M.A.B.M., Furtado, R.A., Senedese, J.M., Bastos, J.K., Tavares, D.C., In vivo protective effect of Copaifera langsdorffii hydroalcoholic extract on micronuclei induction by doxorubicin (2013) J Appl Toxicol, 33, pp. 854-860. , 10.1002/jat.2777, 22611044 Indart, A., Viana, M., Clapés, S., Izquierdo, L., Bonet, B., Clastogenic and cytotoxic effects of lipid peroxidation products generated in culinary oils submitted to thermal stress (2007) Food Chem Toxicol, 45, pp. 1963-1967. , 10.1016/j.fct.2007.04.019, 17573172 Venkatesh, P., Shantala, B., Jagetia, G.C., Rao, K.K., Baliga, M.S., Modulation of Doxorubicin-Induced Genotoxicity by Aegle marmelos in Mouse Bone Marrow: A Micronucleus Study (2007) Integr Cancer Ther, 6, pp. 42-53. , 10.1177/1534735406298302, 17351026 (2010) Farmacopéia Brasileira, Agência Nacional de Vigilância Sanitária - ANVISA/Fundação Oswaldo, , Brasília: Cruz - FIOCRUZ, Brasil Sex differences in the micronucleus test (1986) Mutat Res, 172, pp. 151-163. , Collaborative Study Group for the Micronucleus Test (CSGMT) Zambrano, M.A., Targa, H.J., Rabello-Gay, M.N., Physiological saline solutions as a useful tool in micronucleus and metaphase slide preparations (1982) Stain Technol, 57, pp. 48-49 Lewi, D.M., Hopp, H.E., Escandon, A.S., Sunflower (Helianthus annuus L.) (2006) Methods Mol Biol, 343, pp. 291-297 Heo, J.C., Woo, S.U.K., Kweon, M.A., Park, J.Y., Lee, H.K., Son, M., Rho, J.R., Lee, S.H., Aqueous extract of the Helianthus annuus seed alleviates asthmatic symptoms in vivo (2008) Int J Mol Med Chem, 21, pp. 57-61 Cardoso, C.C., Rodrigues, K.L., Pichara, N.L., Dall'Aglio, R., Fiorini, J.E., Fraschini, F., Diana, G.M., Carvalho, J.C.T., Olio di girasole ozonizzato associate ad acido α-lipoico e a lattobacilli: studio pre-clinico dell'azione antiulcerosa, antinfiammatoria e antibatterica (2004) Farmaci, 28, pp. 97-110 Ricardo, G.A., Zullyt, Z.R., Yilian, L., Hernández, F., Menéndez, S., Efecto del OLEOZON® frente a lesiones gástricas inducidas por indometacina en ratas (Effect of OLEOZON® on gastric lesions induced by indomethacin in rats) (2007) Revista electrónica de Veterinaria, 8, pp. 1-6 Rodrigues, K.L., Cardoso, C.C., Caputo, L.R., Carvalho, J.C., Fiorini, J.E., Schneedorf, J.M., Cicatrizing and antimicrobial properties of an ozonised oil from sunflower seeds (2004) Inflammopharmacology, 3, pp. 261-270 Akihisa, T., Yasukawa, K., Oinuma, H., Kasahara, Y., Kimura, Y., Takase, S., Yamanouchi, S., Tamura, T., Triterpene alcohols from the flowers of Compositae and their anti-inflammatory effects (1996) Phytochemistry, 43, pp. 1255-1260. , 10.1016/S0031-9422(96)00343-3, 8987908 Rojas-Molina, M., Campos-Sanches, J., Analla, M., Serrano, M., Moraga, A.A., Genotoxicity of vegetable cooking oils in the Drosophila wing spot test (2005) Environ Mol Mutagen, 45, pp. 90-95. , 10.1002/em.20078, 15611939 Montero, A.C.R., Carvajal, Y.G., Rodríguez, Z.Z., López, G.F., Mirabal, J.M., Evaluación genotóxica del OLEOZON mediante los ensayos de micronúcleos en medula ósea y sangre periférica de ratón (1998) Revista CENIC: Ciências Biológicas, 29, pp. 200-202 Salerno, J.W., Smith, D.E., The use of sesame oil and other vegetable oils in the inhibition of human colon cancer growth in vitro (1991) Anticancer Res, 11, pp. 209-215 Cognault, S., Jourdan, M.L., Germain, E., Pitavy, R., Morel, E., Durand, G., Bougnoux, P., Lhuillery, C., Effect of an m-linolenic acid-rich diet on rat mammary tumour growth depends on the dietary oxidative status (2000) Nutr Cancer, 36, pp. 33-41. , 10.1207/S15327914NC3601_6, 10798214 Nakayama, M., Ju, H.R., Sugano, M., Hirose, N., Ueki, T., Doi, F., Eynard, A.R., Effect of dietary fat and cholesterol on dimethylbenz [a]-antracene-induced mammary tumorigenesis in Sprague-Dawley rats (1993) Anticancer Res, 13, pp. 691-698 La Vecchia, C., Negri, E., Franceschi, S., Decarli, A., Giacosa, A., Lipworth, L., Olive oil, other dietary fats, and the risk of breast cancer (Italy) (1995) Cancer Cause Control, 6, pp. 545-550 Rao, G.N., Ney, E., Herbert, R.A., Effect of melatonin and linolenic acid on mammary cancer in transgenic mice with c-neu breast cancer oncogen (2000) Breast Cancer Res, 64, pp. 287-296 Burns, C.P., Luttenegger, D.G., Specctor, A.A., Effect of dietary fat saturation on survival of mice with L1210 Leukemia (1978) J Natl Cancer Inst, 61, pp. 513-515 Bartsch, H., Nai, J., Owen, R.W., Dietary polyunsaturated fatty acids and cancer of the breast and colorectum: emerging evidence for their role as risk modifiers (1999) Carcinogenesis, 20, pp. 2209-2218. , 10.1093/carcin/20.12.2209, 10590211 Boovens, J., Engelbrecht, P., Le Roux, S., Louwrens, C.C., Van der Merwe, C.F., Katzeff, I.E., Some effects of the essential fatty acids linoleic acid and alpha-Linolenic acid and of their metabolites gamma-Linolenic acid, arachidonic acid, eicosapentaenoic acid, docosahesaenoic acid, and of prostaglandins A1 and E1 in the proliferation of human osteogenic sarcoma cells in culture (1984) Prostaglandins Leukot Med, 15, pp. 15-33. , 10.1016/0262-1746(84)90053-2, 6089235 Johanning, G.L., Lin, T.Z., Unsaturated fatty acid effects on human breast cancer cell adhesion (1995) Nutr Cancer, 24, pp. 57-66. , 10.1080/01635589509514393, 7491298 Newcomer, L.M., King, I.B., Wicklund, K.G., Stanford, J.L., The association of fatty acids with prostate cancer risk (2001) Prostate, 47, pp. 262-268. , 10.1002/pros.1070, 11398173 Iwado, H., Naito, M., Hayatsu, H., Genotoxicity and antigenotoxicity of air-borne particulates (1991) Mutat Res, 246, pp. 93-103. , 10.1016/0027-5107(91)90110-A, 1986270 Siegel, I., Liu, T.L., Yaghoubzadeh, E., Keskey, T.S., Gleicher, N., Cytotoxic effects of free fatty acids on ascites tumor cells (1987) J Natl Cancer Inst Monographs, 78, pp. 271-277 Bégin, L.R., Clement, P.B., Kirk, M.E., Jothy, S., McCaughey, W.T., Ferenczy, A., Aggressive angiomyxoma of pelvic soft parts: a clinicopathologic study of nine cases (1985) Hum Pathol, 16, pp. 621-628. , 10.1016/S0046-8177(85)80112-X, 3997139 Ramon, J.M., Bou, R., Romea, S., Alkiza, M.E., Jacas, M., Ribes, J., Oromi, J., Dietary fat intake and prostate cancer risk: a case-control study in Spain (2000) Cancer Cause Control, 11, pp. 679-685 DeFlora, S., Ramel, C., Mechanisms of inhibitors of mutagenesis and carcinogenesis: classification and review (1988) Mutat Res, 202, pp. 285-306. , 10.1016/0027-5107(88)90193-5, 3057362 Visioli, F., Bellosta, S., Galli, C., Oleuropein, the bitter principle of olives, enhances nitric-oxide production by mouse macrophages (1998) Life Sci, 62, pp. 541-546. , 10.1016/S0024-3205(97)01150-8, 9464466 Santos, J.H., Graf, U., Reguly, M.L., De Andrade, H.H.R., The synergistic effects of vanillin on recombination predominate over its antigenotoxic action in relation to MMC-induced lesions in somatic cells of Drosophila melanogaster (1999) Mutat Res, 444, pp. 355-365. , 10.1016/S1383-5718(99)00101-1, 10521675 Weisburger, J.H., Can cancer risks be altered by changing nutritional traditions? (1998) Cancer, 83, pp. 1278-1281. , 10.1002/(SICI)1097-0142(19981001)83:7<1278::AID-CNCR2>3.0.CO;2-F, 9762926 Katiyar, S.K., Mohan, R.R., Agarwal, R., Mukhtar, H., Protection against induction of mouse skin papillomas with low and high risk of conversion to malignancy by green tea polyphenols (1997) Carcinogenesis, 18, pp. 497-502. , 10.1093/carcin/18.3.497, 9067548 Indart, A., Viana, M., Grootveld, M.C., Silwood, C.J., Sanchez-Vera, I., Bonet, B., Teratogenic actions of thermally-stressed culinary oils in rats (2002) Free Radic Res Commun Commun, 36, pp. 1051-1058 Al-Shabanah, O.A., Inhibition of adriamycin-induced micronuclei by desferrioxamine in Swiss albino mice (1993) Mutat Res, 301, pp. 107-111. , 10.1016/0165-7992(93)90032-Q, 7678167 Bean, C.L., Armstrong, M.J., Galloway, S.M., Effect of sampling time on chromosome aberration yield for 7 chemicals in Chinese hamster ovary cells (1992) Mutat Res, 265, pp. 31-44. , 10.1016/0027-5107(92)90037-3, 1370241 Delvaeye, M., Verovski, V., De Neve, W., Storme, G., DNA breakage, cytotoxicity, drug accumulation and retention in two human ovarian tumor cell lines AZ224 and AZ364 treated with adriamycin, modulated by verapamil (1993) Anticancer Res, 13, pp. 1533-1538 Dhawan, A., Kayani, M.A., Parry, J.M., Parry, E., Anderson, D., Aneugenic and clastogenic effects of doxorubicin in human lymphocytes (2003) Mutagenesis, 18, pp. 487-490. , 10.1093/mutage/geg024, 14614182 Jagetia, G.C., Nayak, V., Effect of doxorubicin on cell survival and micronuclei formation in HeLa cells exposed to different doses of gamma-radiation (2000) Strahlenther Onkol, 176, pp. 422-428. , 10.1007/PL00002351, 11050916 Vidhya, N., Devraj, S.N., Antioxidant effect of eugenol in rat intestine (1999) Ind J Exp Biol, 37, pp. 1192-1195