Artículos de revistas
Molecular Characterization Of Hemoglobin α-d Chains From Geochelone Carbonaria And Geochelone Denticulata Land Turtles
Registro en:
Comparative Biochemistry And Physiology - B Biochemistry And Molecular Biology. , v. 134, n. 2, p. 389 - 395, 2003.
10964959
10.1016/S1096-4959(02)00289-0
2-s2.0-0037304903
Autor
Melo M.B.
Bordin S.
Duarte A.S.S.
Ogo S.H.
Torsoni M.A.
Saad S.T.O.
Costa F.F.
Institución
Resumen
In order to help elucidate the evolution of α-globins, the complete cDNA and amino acid sequences of Geochelone carbonaria and Geochelone denticulata land turtles α-D chains have been described. In G. carbonaria, the cDNA is 539 bp with ATG start codon located at position 46, TGA stop codon at position 469 and AATAAA polyadenylation signal at position 520. In G. denticulata, the cDNA is 536 bp with ATG start codon located at position 46, TGA stop codon at position 469 and AATAAA polyadenylation signal at position 517. Both cDNAs codify 141 amino acid residues, differing from each other in only four amino acid residues. When comparing with human Hb α-chain, alterations in important regions can be noted: α110 Ala-Gly, α114 Pro-Gly, α117 Phe-Tyr and α122 His-Gln. There is a high homology between the amino acids of these turtles when compared with chicken α-D chains, progressively decreasing when compared with human, crocodile, snake, frog and fish α-chains. Phylogenetic analysis of α-D chains shows that those of turtles are closer to those of birds than to snakes and lizards. © 2002 Elsevier Science Inc. All rights reserved. 134 2 389 395 Baldwin, J.M., The structure of human carbonmonoxy haemoglobin at 2.7 A resolution (1980) J. Mol. Biol., 136, pp. 103-128 Bordin, S., Meza, N.A., Saad, S.T.O., Ogo, S.H., Costa, F.F., CDNA-derived amino-acid sequence of a land turtle (Geochelone carbonaria) β-chain hemoglobin (1997) Biochem. Mol. Biol. Int., 42, pp. 255-260 Bunn, H.F., Forget, B.G., Hemoglobin structure (1986) Hemoglobin: Molecular, Genetic and Clinical Aspects, pp. 13-35. , Philadelphia, PA: W.B. Saunders Company Fushitani, K., Higashiyama, K., Moriyama, E.M., Imai, K., Hosokawa, K., The amino acid sequences of two α chains of hemoglobins from Komodo dragon Varanus komodoensis and phylogenetic relationships of amniotes (1996) Mol. Biol. Evol., 13, pp. 1039-1043 Gorr, T.A., Mable, B.K., Kleinschmidt, T., Phylogenetic analysis of reptilian hemoglobins: Trees, rates, and divergences (1998) J. Mol. Evol., 47, pp. 471-485 Hashimoto, M., Ishimori, K., Imai, K., Site-directed mutagenesis in hemoglobin: Functional and structural study of the intersubunit hydrogen bond of threonine-38(C3)alpha at the alpha 1-beta 2 interface in human hemoglobin (1993) Biochemistry, 32, pp. 13688-13695 Komiyama, N.H., Miyazaki, G., Tame, J., Nagai, K., Transplanting a unique allosteric effect from crocodile into human haemoglobin (1995) Nature, 37, pp. 244-246 Mylvaganam, S.E., Bonaventura, C., Bonaventura, J., Getzoff, E.D., Structural basis for the root effect in haemoglobin (1996) Nature Struct. Biol., 3, pp. 275-283 Petruzzelli, R., Aureli, G., Lania, A., Galtieri, A., Desideri, A., Giardina, B., Diving behaviour and haemoglobin function: The primary structure of the α- and β-chains of the sea turtle (Caretta caretta) and its functional implications (1996) Biochem. J., 316, pp. 959-965 Rawn, J.D., Amino acids and the primary structure of proteins (1989) Biochemistry, p. 54. , L.P. Daisy, K.C. Hodgin, T.L. O'Quin, S. Olsen, & J.A. Swan. Burlington, NC: Neil Patterson Publishers Rücknagel, K.P., Reischl, E., Braunitzer, G., Hemoglobins of reptiles. Expression of alpha-D-genes in the turtles, Chrysemys picta bellii and Phrynops hilarii (Testudines) (1984) Hoppe-Seyler's Z. Physiol. Chem., 365, pp. 1163-1171 Rücknagel, K.P., Braunitzer, G., The primary structure of the major and minor hemoglobin component of adult western painted turtle (Chrysemys picta bellii) (1988) Biol. Chem. Hoppe-Seyler, 369, pp. 123-131 Saitou, N., Nei, M., The neighbor-joining method: A new method for reconstructing phylogenetic trees (1987) Mol. Biol. Evol., 4, pp. 406-425 Shishikura, F., Takami, K., The amino acid sequences of the alpha- and beta-globin chains of hemoglobin from the aldabra giant tortoises, Geochelone gigantea (2001) Zool. Sci., 18, pp. 515-526 Shishikura, F., The primary structure of hemoglobin D from the Aldabra giant tortoise, Geochelone gigantea (2002) Zool. Sci., 19, pp. 197-206 Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequences weighting, positions-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res., 22, pp. 4673-4680 Torsoni, M.A., Ogo, S.H., Oxygenation properties of hemoglobin from the turtle Geochelone carbonaria (1995) Braz. J. Med. Biol. Res., 28, pp. 1129-1131 Torsoni, M.A., Viana, R.I., Stoppa, G.R., Barros, B.F., Cesquini, M., Ogo, S.H.J., Effect of thiol reagents on functional properties and heme oxidation in the hemoglobin of Geochelone carbonaria (1996) Biochem. Mol. Biol. Int., 40, pp. 355-364 Torsoni, M.A., Souza-Torsoni, A., Ogo, S.H., Involvement of available SH groups in the heterogeneity of hemoglobin from the tortoise Geochelone carbonaria (1998) Biochem. Mol. Biol. Int., 44, pp. 851-860 Torsoni, M.A., Ogo, S.H., Hemoglobin-sulfhydryls from tortoise (Geochelone carbonaria) can reduce oxidative damage induced by organic hydroperoxide in erythrocyte membrane (2000) Comp. Biochem. Physiol. B. Biochem. Mol. Biol., 126, pp. 571-577 Zhang, Y., Frohman, M.A., CDNA library protocols (1997) Methods in Molecular Biology, vol. 69, pp. 61-87. , I.G. Cowell, & C.A. Austin. Totowa, NJ: Humana Press Inc