The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly affects the pectinesterase, and endo- and exopolygalacturonase activities. Pectin, glucose and saccharose, when added to the culture medium in high concentrations, exhibited a repression effect on all the analyzed enzymes.Foi avaliado o efeito de diferentes fontes de carbono na atividade de pectinesterease, endo - e exo-polygalacturonase de Aspergillus japonicus 586, em meio líquido (soluções de Manachini) suplementado com diferentes concentrações de substratos. O meio de cultura foi inoculado com 5 x 10(6) esporos/ml e mantido sob agitação (140 rpm), a 30°C, durante 120 h. A avaliação das enzimas foi realizada a cada 24 h após filtração. O extrato cru de A. japonicus 586 mostrou que as melhores atividades enzimáticas foram obtidas na presença de 0,5% pectina (pectinestarease), 0,2% pectina e 0,2% glicerol (endopoligalacturonase) e 0,5% pectina associada a 0,5% glicose (exopoligalacturonase). Diferentes concentrações de fontes de carbono, isoladas ou associadas afetaram significativamente as atividades de polinesterase, endo- e exopoligalacturonase. Quando adicionado pectina, glicose e sacarose em altas concentrações, ao meio de cultura, observou-se um efeito de repressão de todas as enzimas analisadas.286290