| dc.creator | George-Nascimento, Mario | |
| dc.creator | Oliva, Marcelo | |
| dc.creator | Valdivia, Isabel M. | |
| dc.creator | Cárdenas, Leyla | |
| dc.creator | Gonzalez, Karen | |
| dc.creator | Guiñez, Ricardo E. | |
| dc.creator | Cuello, Diego | |
| dc.date | 2015-12-01T15:27:28Z | |
| dc.date | 2015-12-01T15:27:28Z | |
| dc.date | 2010 | |
| dc.identifier | Parasitology Research 106 | |
| dc.identifier | 1432-1955 | |
| dc.identifier | http://repositoriodigital.ucsc.cl/handle/25022009/652 | |
| dc.description | Artículo de publicación ISI | |
| dc.description | The mussel Perumitylus purpuratus has been described as the first intermediate host for the digenea Proctoeces lintoni (Fellodistomidae) in the Chilean coast. The study of more than 3000 specimens of P. purpuratus, taken off northern Chile revealed the absence of sporocysts. Experimental infection of mussels with eggs obtained from the known host for P. lintoni was unsuccessful. We analyze the V4 region of the SSU rRNA of living sporocysts and cercariae obtained from P. purpuratus from central Chile in order to confirm the proposed life cycle for this digenea. Our results demonstrated that sporocysts and cercariae obtained from P. purpuratus do not belong to P. lintoni but to an undescribed digenea from the Chilean coast. The most studied digenea from marine organisms along the Chilean coast (18ºS to 56ºS) is by far Proctoeces lintoni. This digenea species is found as a gonadic parasite of keyhole limpets (Fissurella spp., Archaeogastropoda) and in the intestine of the clingfish Syciases sanguineus (Gobiesocidae). Recently Loot et al. (2005; 2008) suggest a three-host life cycle for this parasite. In summary, the first intermediate host is the mussel Perumytilus purpuratus, in which the parasite produces cercariae. Cercariae leave the mussels to infect the gonads of the keyhole limpet Fissurella spp. the second intermediate host, where they develop into the metacercariae stage. The infected limpet is then ingested by the definitive host, the clingfish S. sanguineus, wherein the parasite reaches the sexual maturity beginning egg production. The proposed life cycle (Loot et al. 2005; 2008) was based on bibliographic references related to the well-known presence of P. lintoni in keyhole limpets and clingfish (George-Nascimento et al. 1998; Oliva and Huaquin 2000) and field and laboratory observations of sporocysts producing cercariae in the mussel P. purpuratus at two localities at central Chile (Las Cruces 33º31′S, 71º38′W and El Quisco 33º24′S, 71º42′W; Aldana 2007). Prevalence of infection (percentage of infected individuals) can reach 100% in Fissurella crassa in central Chile (Loot et al. 2005) and northern Chile (Oliva and Huaquin 2000), more than 80% in S. sanguineus but no more than 4.1% in P. purpuratus (Loot et al. 2005). | |
| dc.language | en | |
| dc.publisher | Springer | |
| dc.rights | Atribucion-Nocomercial-SinDerivadas 3.0 Chile | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
| dc.source | http://goo.gl/ANvi0G | |
| dc.subject | Proctoeces lintoni | |
| dc.subject | Fellodistomidae | |
| dc.subject | Chile | |
| dc.title | Molecular and experimental evidence refuse the life cycle of Proctoeces lintoni (Fellodistomidae) in Chile | |
| dc.type | Artículos de revistas | |
