| dc.creator | Illanes, Andrés | |
| dc.creator | Wilson, Lorena | |
| dc.creator | Altamirano, Claudia | |
| dc.creator | Cabrera, Z. | |
| dc.creator | Álvarez, L. | |
| dc.creator | Aguirre Céspedes, Carolina | |
| dc.date | 2015-11-24T19:06:56Z | |
| dc.date | 2015-11-24T19:06:56Z | |
| dc.date | 2007 | |
| dc.identifier | Enzyme and Microbial Technology 40 | |
| dc.identifier | 0141-0229 | |
| dc.identifier | http://repositoriodigital.ucsc.cl/handle/25022009/491 | |
| dc.description | Artículo de publicación ISI | |
| dc.description | The kinetically controlled synthesis of cephalexin in ethylene glycol was previously optimized at moderate substrate concentrations obtaining yields close to stoichiometric. A study is now presented on the production of cephalexin at very high substrates concentrations, up to 750 mM acyl donor, with immobilized and cross-linked enzyme aggregates (CLEA) of penicillin acylase. Since conversion yield close to 100% was already obtained, attention was given to productivity under the hypothesis that increasing substrates concentration will produce a substantial increase in productivity without reducing yield. An increase of 29 times in volumetric productivity and 4.5 times in specific productivity was obtained with PGA-450 with respect to the results obtained at moderate substrates concentrations (below 100 mM acyl donor). Volumetric productivity was lower for CLEA than for PGA-450, but specific productivity was almost the same for both. Sequential batch reactor operations were conducted to assess the biocatalyst operational stability and global productivity, considering one half-life as biocatalyst life cycle. Under such criterion, 40.1 and 135.5 g of cephalexin/g of biocatalyst were obtained for PGA-450 and CLEA, respectively. Yields remained close to 100% during the whole cycle. These are very good values which can be improved by optimizing the biocatalyst replacement criterion. | |
| dc.language | en | |
| dc.publisher | ELSEVIER | |
| dc.source | https://pure.pucv.cl/es/publications/production-of-cephalexin-in-organic-medium-at-high-substrate-conc | |
| dc.subject | Penicillin acylase | |
| dc.subject | CLEA | |
| dc.subject | Cephalexin | |
| dc.subject | Organic cosolvents | |
| dc.title | Production of cephalexin in organic medium at high substrate concentrations with CLEA of penicillin acylase and PGA-450 | |
| dc.type | Artículos de revistas | |
