| dc.contributor | | en-US |
| dc.creator | Madera Anaya, Meisser Vidal | |
| dc.creator | Suárez Causado, Amileth | |
| dc.date | 2018-01-10 | |
| dc.date.accessioned | 2018-03-16T16:00:11Z | |
| dc.date.available | 2018-03-16T16:00:11Z | |
| dc.identifier | http://ojs.unam.mx/index.php/rom/article/view/63050 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1204294 | |
| dc.description | Objective: To standardize an RNA (ribonucleic acid) extraction protocol in children’s saliva specimens. Methods: The study was conducted on saliva specimens from 60 children who participated in the study with their parents’ authorization. Comparison of two RNA extraction methods was established; methods assessed concentration, quality and yield. Moreover, genic expression of GUSB gene was achieved with RT-PCR (reverse transcriptasepolymerase chain reaction). Data were analyzed through measurements of central tendency and dispersion, frequencies and percentages. T-Student and χ2 tests were used in order to differentiate between both methods (p < 0.05). Results: Analysis of cell amounts per saliva ml revealed a mean of 564,977.8 (SD = 246,678.6); a RNA in children’s saliva extraction method was standardized, specifically the method using RNeasy® Protect Saliva Mini Kit Qiagen exhibited better characteristics of RNA concentration (p = 0.0000) and yield (p = 0.0000) when compared to the method using QIAzol®; no RNA (p = 0.146) quality differences were found. Conclusions: RNA could be extracted from children’s saliva specimens, therefore, it is suggested to use saliva for the molecular analysis of different oral and systemic diseases. | en-US |
| dc.format | application/pdf | |
| dc.language | spa | |
| dc.publisher | Facultad de Odontología | es-ES |
| dc.relation | http://ojs.unam.mx/index.php/rom/article/view/63050/55428 | |
| dc.rights | ##submission.copyrightStatement## | es-ES |
| dc.source | Revista Odontológica Mexicana; Vol 21, No 4 Ing | es-ES |
| dc.source | 1870-199X | |
| dc.subject | | en-US |
| dc.subject | Saliva, RNA, transcriptome, biomarkers | en-US |
| dc.title | Evaluation of two RNA extraction methods in children’s saliva | en-US |
| dc.type | Artículos de revistas | |
| dc.type | Artículos de revistas | |
