dc.contributoren-US
dc.creatorArroyo Cervantes, Roberto
dc.creatorCuin Macedo, Sergio Iván
dc.creatorCalderón Rojas, Benigno Miguel
dc.creatorRodríguez Zaragoza, Diana Ened
dc.creatorRuiz Reyes, Héctor
dc.date2017-01-26
dc.date.accessioned2018-03-16T15:59:38Z
dc.date.available2018-03-16T15:59:38Z
dc.identifierhttp://ojs.unam.mx/index.php/rom/article/view/57725
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1204249
dc.descriptionIntroduction: Sodium hypochlorite (NaOCl) is the chemical agent most frequently used as irrigation solution during endodontic therapy. When extruded to periapical tissue, it is highly toxic. In endodontics, hemolysis caused by NaCOl has been proven using different models, nevertheless, there is little or no evidence of morphological alterations in the cellular membrane of erythrocytes. Objective: To propose an experimental model which might allow to assess morphological alterations suffered by erythrocytes when they are exposed to NaOCl used in the dental practice by means of high resolution scanning electron microscopy (SEM). Materials and methods: In the present study, 20 mL of peripheral blood were obtained and deposited in tubes with EDTA (ethylenediaminetetraacetic acid) anticoagulant. Rinses were conducted with a phosphate buffer solution (Evan’s solution). Several dilutions of the erythrocyte sample were prepared (1:1, 1:2, 1:4, 1:8 and 1:16); 100 μL of each of these dilutions was obtained to be then confronted with 100 μL of dental use 5.25% NaOCl (Viarzoni-T, Medental®); 0.5 μL of these samples were taken to then be deposited in a sample holder made of Zn-Cu alloy which was subjected to a process of Cu ion metallization bath, following the old Spluttering method. Microphotographs were obtained with SEM. Results: Erythrocytes with alteration type anisocytosis and poikilocytosis (stomatocytes, elliptocytes and discocytes) were observed. Some structural characteristics of NaOCl crystals were equally observed. Conclusion: This experimental model allowed assessment of morphological changes experienced by erythrocytes when exposed to 5.25% NaOCl.en-US
dc.formatapplication/pdf
dc.languagespa
dc.publisherFacultad de Odontologíaes-ES
dc.relationhttp://ojs.unam.mx/index.php/rom/article/view/57725/51166
dc.rights##submission.copyrightStatement##es-ES
dc.sourceRevista Odontológica Mexicana; Vol 20, No 4-INGes-ES
dc.source1870-199X
dc.subjecten-US
dc.subjectExperimental model, sodium hypochlorite, erythrocytes.en-US
dc.titleProposal for experimental in vitro model to assess morphological alterations in erythrocytes exposed to 5.25% NaOClen-US
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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