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Differences in preservation of canine chilled semen using simple sperm washing, single-layer centrifugation and modified swim-up preparation techniques
(Csiro Publishing, 2016-04)
This study compared the efficacy of simple sperm washing (SW), single-layer centrifugation (SLC) and modified swim-up (SU) techniques in the preparation of dog spermatozoa for cooling. Eighteen ejaculates, collected from ...
Supplementing a skimmed milk-egg yolk-based extender with L-carnitine helps maintain the motility, membrane integrity and fertilizing capacity of chilled ram sperm
(2020)
This study examines the effect of L-carnitine (LC) on chilled ram semen stored for up to 96 hr. Semen samples were collected, placed in a skimmed milk + 6% egg yolk extender, pooled, aliquoted and diluted with the same ...
In vitro sperm penetration through the zona pellucida of immature and in vitro matured oocytes using fresh, chilled and frozen canine semen
(ELSEVIER SCIENCE BV, 2009-01)
The aim of this study was to evaluate the effect of sperm cryopreservation and the maturation state of the
oocyte on the time course of canine gamete interaction during co-culture for periods of 1–10 h. Semen samples
were ...
Effect of milk extender supplemeted with vitamin E analog in chilled canine semen
(Wiley-Blackwell, 2012-08-01)
Influence of sperm filtration and the addition of glycerol to UHT skimmed milk- and TEST-based extenders on the quality and fertilizing capacity of chilled ram sperm
(2019)
The poor fertility of ram semen stored chilled for long periods has encouraged the development of protocols designed to improve the kinetic vigour and cervical barrier-crossing capacity of sperm. The present work evaluated ...
Efecto de la refrigeración sobre la motilidad, integridad de membrana acrosomal y reacción acrosomal en espermatozoides caninos
(Universidad Nacional Mayor de San Marcos, 2005)
Preservation methods of spermatozoa using refrigeration have been developed for
canine sperm; however, the rate of fertilization is still low, possibly due to cell damage for
unknown reasons. The objective of this study ...
Effect of L-carnitine in a skimmed milk based-diluent on membranes preservation and kinetic activity of ram sperm under chilled conditions
(Asociación Interprofesional para el Desarrollo Agrario, 2019)
The synergic effect of L-carnitine (LC) and skimmed milk base-extender (UHT) on the kinetic parameters and membranes integrity of cold-stored ram sperm was evaluated. For this purpose, twelve pools of thirty-six semen ...
Effect of milk extender supplemeted with vitamin E analog in chilled canine semen
(Wiley-Blackwell, 2014)
Effect of milk extender supplemeted with vitamin E analog in chilled canine semen
(Wiley-Blackwell, 2012-08-01)