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Challenges and trends in bioseparations
(JOHN WILEY, 2008-02)
In this paper the issues and challenges presented 15 years ago for performing efficient separation processes for recombinant proteins are revised and discussed. Competitive advantage in production was seen as not only ...
Precipitation with poly acrylic acid as a trypsin bioseparation strategy
(Elsevier, 2009-09)
Precipitation of enzymes with reversible soluble-insoluble polymers is a simple approach which can be easily scaled up. This work reports investigations aiming at verifying the existence of specific interactions and complex ...
Polyelectrolytes–protein complexes: a viable platform in the downstream processes of industrial enzymes at scaling up level
(John Wiley & Sons Ltd, 2016-12)
Complexation between proteins and polyelectrolytes through electrostatic interactions gives either soluble or non-soluble complexes, leading to phase separation. The formation and the stability of these complexes are ...
Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation
(Elsevier, 2016-07)
The precipitation of endoglucanase from Aspergillus niger with synthetic and natural electrically charged polymers -poly vinyl sulfonate (PVS) and chitosan (CHS)- was characterized and applied to a simple method of ...
Adsorption of amoxicillin on chitosan beads: Kinetics, equilibrium and validation of finite bath models
(Elsevier Science SaLausanneSuíça, 2005)
Interaction of tannase from Aspergillus niger with polycations applied to its primary recovery
(Elsevier Science, 2012-07)
Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich ...
Recovery of casein-derived peptides with in vitro inhibitory activity of angiotensin converting enzyme (ACE) using aqueous two-phase systems
(Journal of Chromatography B, 2018)