Articulo
Isolation, purification and preliminary characterization of cryophilic proteases of marine origin
BIOSEPARATION (DORDRECHT. PRINT);
BIOSEPARATION (DORDRECHT. PRINT)
Registro en:
0
D01I1031
D01I1031
WOS:000176894100012
0923-179X
Autor
Asenjo-De Leuze, Juan
Andrews-Farrow, Barbara
Barria , C.
Salamanca, M.
Institución
Resumen
The isolation and preliminary characterization of a trypsin-like protease with high activity at 20 degreesC is described. This protease was isolated from Antarctic krill (Euphasia superba) by a two-step chromatography process and the use of zymogram analyses. The protease has a molecular weight of 30 kDa and a pI of 4.1. Its specific activity at 20 degreesC on BAPNA is 0.5 U/mg. 0 3 FONDEF 0 0 4 FONDEF 10