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Single molecule studies by optical tweezers: folding and unfolding of glucokinase from Thermococcus litoralis
(Universidad de Chile, 2011)
En esta tesis utilizamos la técnica de pinzas opticas para investigar el efecto que
ejerce el sustrato sobre la estabilidad mecánica de la enzima glucoquinasa de la arquea
hipertermofila Thermococcus litoralis que crece ...
Single molecule studies by optical tweezers: folding and unfolding of glucokinase from Thermococcus litoralis
(Universidad de Chile, 2011)
Single-molecule manipulation has increasingly become a useful methodology for the study of macromolecular dynamics including unfolding-refolding transitions. In this study we use the optical tweezers to investigate the ...
Dissecting the functional roles of the conserved nxxe and hxe motifs of the adp-dependent glucokinase from thermococcus litoralis
(2015)
The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TIGK) relies on the highly conserved motifs NXXE (i.e. Asn-Xaa-Xaa-Glu) and HXE (i.e. His-Xaa-Glu). Site-directed mutagenesis of residues Glu279 ...
Dissecting the functional roles of the conserved NXXE and HXE motifs of the ADP-dependent glucokinase from Thermococcus litoralis
(Elsevier, 2015)
The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TlGK) relies on the
highly conserved motifs NXXE (i.e. Asn-Xaa-Xaa-Glu) and HXE (i.e. His-Xaa-Glu). Site-directed mutagenesis
of residues Glu279 ...
Crystal Structure, SAXS and Kinetic Mechanism of Hyperthermophilic ADP-Dependent Glucokinase from Thermococcus litoralis Reveal a Conserved Mechanism for Catalysis
(2013-06)
ADP-dependent glucokinases represent a unique family of kinases that belong to the ribokinase superfamily, being present
mainly in hyperthermophilic archaea. For these enzymes there is no agreement about the magnitude of ...