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Identifying Sequential Substrate Binding at the Single-Molecule Level by Enzyme Mechanical Stabilization
(ACS Publications, 2015)
Enzyme-substrate binding is a dynamic process intimately coupled to protein structural changes, which in turn changes the unfolding energy landscape. By the use of single-molecule force spectroscopy (SMFS), we characterize ...
ATP-binding cassette exporters: Structure and mechanism with a focus on P-glycoprotein and MRP1
(Bentham Science Publishers, 2019-09)
Background: Proteins that belong to the ATP-binding cassette superfamily include transporters that mediate the efflux of substrates from cells. Among these exporters, P-glycoprotein and MRP1 are involved in cancer multidrug ...
Lipid Metabolizing Enzyme Activities Modulated by Phospholipid Substrate Lateral Distribution
(2011)
Biological membranes contain many domains enriched in phospholipid lipids and there is not yet clear explanation about how these domains can control the activity of phospholipid metabolizing enzymes. Here we used the surface ...
Subsites of trypsin active site favor catalysis or substrate binding
(Elsevier B.V., 2002-01-11)
Enzymes enhance chemical reaction rates by lowering the activation energy, the energy barrier of the reaction leading to products. This occurs because enzymes bind the high-energy intermediate of the reaction (the transition ...
Intrinsically disordered linkers impart processivity on enzymes by spatial confinement of binding domains
(Multidisciplinary Digital Publishing Institute, 2019-05)
(1) Background: Processivity is common among enzymes and mechanochemical motors that synthesize, degrade, modify or move along polymeric substrates, such as DNA, RNA, polysaccharides or proteins. Processive enzymes can ...
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates
(Instituto de Ci??ncias da Sa??de/ Universidade Federal da Bahia, 2004-01)
Characterization of temperature dependent and substrate-binding cleft movements in Bacillus circulans family 11 xylanase: A molecular dynamics investigation
(ELSEVIER SCIENCE BV, 2009)
Background: Xylanases (EC 3.2.1.8) hydrolyze xylan, one of the most abundant plant polysaccharides found in nature, and have many potential applications in biotechnology. Methods: Molecular dynamics simulations were used ...
Spectroscopic and thermodynamic measurements of nucleotide-induced changes in the human 70-kDa heat shock cognate protein
(Elsevier Science IncNew YorkEUA, 2006)
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine
(2005-10-01)
Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of nucleosides and deoxynucleosides, generating ribose 1-phosphate and the purine base, which is an important step of purine catabolism pathway. ...
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine
(2005-10-01)
Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of nucleosides and deoxynucleosides, generating ribose 1-phosphate and the purine base, which is an important step of purine catabolism pathway. ...