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STED microscopy of living cells: New frontiers in Membrane and Neurobiology
(Wiley, 2013-04-23)
Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current ...
STED microscopy of living cells--new frontiers in membrane and neurobiology
(Wiley, 2013)
Abstract: Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review ...
STED nanoscopy with wavelengths at the emission maximum
(IOP Publishing, 2016-08)
Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and ...
Novel red fluorophores with superior performance in STED microscopy
(Springer Open, 2012-09)
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including ...
Recent applications of superresolution microscopy in neurobiology
(Elsevier, 2014-05)
Chemical synapses in brain are structural differentiations where excitatory or inhibitory signals are vectorially transmitted between two neurons. Excitatory synapses occur mostly on dendritic spines, submicron sized ...
Super-resolution Imaging of Energy Transfer by Intensity-Based STED-FRET
(American Chemical Society, 2021-03)
Förster resonance energy transfer (FRET) imaging methods provide unique insight into the spatial distribution of energy transfer and (bio)molecular interaction events, though they deliver average information for an ensemble ...
A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging
(American Chemical Society, 2016-06)
A coumarin fluorophore and an oxazine photochrome can be integrated within the same molecular skeleton and connected covalently to a secondary antibody. Illumination of the antibody-dyad conjugate at an appropriate activation ...
Astrocyte-to-neuron communication through integrin-engaged Thy-1/CBP/Csk/Src complex triggers neurite retraction via the RhoA/ROCK pathway
(Elsevier B.V., 2017)
© 2016 Elsevier B.V. Two key proteins for cellular communication between astrocytes and neurons are αvβ3 integrin and the receptor Thy-1. Binding of these molecules in the same (cis) or on adjacent (trans) cellular membranes ...
Statistical Analysis of High-Resolution Light Microscope Images Reveals Effects of Cytoskeleton-Disrupting Drugs on the Membrane Organization of the Nicotinic Acetylcholine Receptor
(Springer, 2010-06)
Extensive evidence supports the notion that the cytoskeleton participates in the immobilization and mem- brane clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Stimulated emission ...
Quantitative expansion microscopy for the characterization of the spectrin periodic skeleton of axons using fluorescence microscopy
(Nature Publishing Group, 2020-02)
Fluorescent nanoscopy approaches have been used to characterize the periodic organization of actin,spectrin and associated proteins in neuronal axons and dendrites. This membrane-associated periodicskeleton (MPS) is conserved ...