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Reverse Transcription Errors and RNA-DNA Differences at Short Tandem Repeats
(2016-07-12)
Transcript variation has important implications for organismal function in health and disease. Most transcriptome studies focus on assessing variation in gene expression levels and isoform representation. Variation at the ...
Reverse Transcription Errors and RNA-DNA Differences at Short Tandem Repeats
(2016-07-12)
Transcript variation has important implications for organismal function in health and disease. Most transcriptome studies focus on assessing variation in gene expression levels and isoform representation. Variation at the ...
Identification of two putative reference genes from grapevine suitable for gene expression analysis in berry and related tissues derived from RNA-Seq data
(BMC, 2013-12)
Background: Data normalization is a key step in gene expression analysis by qPCR. Endogenous control genes are used to estimate variations and experimental errors occurring during sample preparation and expression measurements. ...
Inter-laboratory ring trial to evaluate real-time reverse transcription polymerase chain reaction methods used for detection of infectious pancreatic necrosis virus in Chile
(Pontificia Universidad Catolica de Valparaiso, 2017)
© 2017 Background Infectious Pancreatic Necrosis Virus (IPNV) is the etiological agent of a highly contagious disease that affects salmonids. In Chile, the second worldwide salmon producer, IPNV causes great economic loss ...
Reference gene selection for quantitative PCR in liver, skeletal muscle, and jejunum of Bos indicus cattle
(Sociedade Brasileira de Zootecnia (SBZ), 2022)
Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos
(BMCCENTAUROLondres, Inglaterra, 2023)
High Rates of Human Immunodeficiency Virus Type 1 Mutational Profiles by Single-Genome Amplification after 48-Hour Propagation in Peripheral Blood Mononuclear Cells at Different Levels of Cell Activation
(Karger, 2014-01-01)
Human immunodeficiency virus type 1 (HIV-1) genetic diversity is one of the most important features of HIV-1 infections and the result of error accumulation during reverse transcription and of high viral turnover. HIV-1 ...
Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
(Czech Academy of Sciences. Institute of Experimental Botany, 2018-01)
Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, ...
Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
(Springer, 2018)
Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, ...