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Expression and purification of human respiratory syncytial virus recombinant fusion protein
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2008)
The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The ...
Expression and purification of human respiratory syncytial virus recombinant fusion protein
(Academic Press Inc. Elsevier B.V., 2008-12-01)
The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The ...
Expression and purification of human respiratory syncytial virus recombinant fusion protein
(Academic Press Inc. Elsevier B.V., 2008-12-01)
The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The ...
New tools for recombinant protein production in Escherichia coli : A 5‐year update
(John Wiley & Sons Inc, 2019-07)
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, ...
Inhibition of the Hantavirus Fusion Process by Predicted Domain III and Stem Peptides from Glycoprotein Gc
(2016)
Hantaviruses can cause hantavirus pulmonary syndrome or hemorrhagic fever with renal syndrome in humans. To enter cells, hantaviruses fuse their envelope membrane with host cell membranes. Previously, we have shown that ...
TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the FliC flagellin of Salmonella Typhimurium
(Instituto Oswaldo Cruz, Ministério da Saúde, 2011)
Recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and Salmonella enterica serovar Typhimurium ...
Recombinant protein expression in microbial systems
(Frontiers, 2014-07)
The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction ...