Buscar
Mostrando ítems 1-10 de 3127
Experimental validation of the predictions of a mathematical model for protein purification and tag selection
(2010)
To improve downstream processing, protein engineering can be used to modify particular properties of a protein, such as specific affinity, charge, or hydrophobicity. The most common modification involves the peptide tags ...
Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein
(2018-07-02)
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker; however, its widespread use is limited by its purification costs. Alternative low-cost purification techniques can overcome this issue. ...
Synergy of DNA-bending nucleoid proteins and macromolecular crowding in condensing DNA
(2007-10-01)
Many prokaryotic nucleoid proteins bend DNA and form extended helical protein-DNA fibers rather than condensed structures. On the other hand, it is known that such proteins (such as bacterial HU) strongly promote DNA ...
Synergy of DNA-bending nucleoid proteins and macromolecular crowding in condensing DNA
(2007-10-01)
Many prokaryotic nucleoid proteins bend DNA and form extended helical protein-DNA fibers rather than condensed structures. On the other hand, it is known that such proteins (such as bacterial HU) strongly promote DNA ...
Expression and purification of human respiratory syncytial virus recombinant fusion protein
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2008)
The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The ...
Expression and purification of human respiratory syncytial virus recombinant fusion protein
(Academic Press Inc. Elsevier B.V., 2008-12-01)
The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The ...
Expression and purification of human respiratory syncytial virus recombinant fusion protein
(Academic Press Inc. Elsevier B.V., 2008-12-01)
The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The ...
Determination of the primary structure (amino acid sequence) of the polyhedryn protein from baculovirus Anticarcia (AgMNPV); final report
(Instituto Interamericano de Cooperación para la Agricultura (IICA), 1989)
The activity refers to the research and applications of genetic resources, biotechnology and biologic control of plagues, diseases and weeds, especially, determination of the primary structure of the polyhedryn protein ...