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Aumento en la transfección genética de la línea celular PC-12 mediada por fármacos que aumentan la fusión celular e inhiben la función lisosomal
(25/02/2010)
ABSTRACT: Gene therapy can be defined as the introduction of genetic material into cells with the objective of altering the course of a disease. Efficient and safe genetic vehicles are one of the fundamental requirements ...
Lipid-based transfection reagents can interfere with cholesterol biosynthesis
(Academic Press Inc Elsevier Science, 2016-02)
Lipid-based transfection reagents are widely used for delivery of small interfering RNA into cells. We examined whether the commonly used commercial transfection reagents DharmaFECT-4 and Lipofectamine 2000 can interfere ...
Chitosan derivatives for gene transfer: effect of phosphorylcholine and diethylaminoethyl grafts on the in vitro transfection efficiency
(2016-11-01)
The purpose of this work was to improve the functional properties of chitosan for gene transfer by inserting phosphorylcholine (PC) and diethylaminoethyl (DEAE) groups into the main chain. A series of derivatives containing ...
Estudios de fármacos que aumentan la transfección genética mediada por lipofección
(30/06/2009)
ABSTRACT: Gene therapy is a strategy for the transference of genes into specific cells from animals or humans, in order to correct genetic alterations that cause diseases. Currently, gene therapy is in experimental phase, ...
Determinación de la eficiencia de transfección de un plásmido portador del gen de proteína de fluorescencia verde en células madres mesenquimales bovinas obtenidas desde médula ósea fetal
(Universidad de Chile, 2015)
El potencial de autorenovación y diferenciación multilinaje ha generado expectativas respecto del uso de células madres mesenquimales (CMM) para ingeniería tisular y medicina regenerativa. En este sentido es relevante ...
Aplicação de derivados de quitosana como agentes de transfecção para transferência gênica não viral
(Universidade Estadual Paulista (Unesp), 2013-09-13)
One possible strategy to improve the efficiency of chitosan as a non viral vector is the modification on the polycation with the additions of hydrophilic, pH-sensitive and selective groups for the target cells, without ...