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The CRISPR toolbox for the gram-positive model bacterium Bacillus subtilis
(2021-01-01)
CRISPR has revolutionized the way we engineer genomes. Its simplicity and modularity have enabled the development of a great number of tools to edit genomes and to control gene expression. This powerful technology was first ...
Validation of reference genes for gene expression studies in the honey bee, Apis mellifera, by quantitative real-time RT-PCR
(SPRINGER FRANCE, 2008)
For obtaining accurate and reliable gene expression results it is essential that quantitative real-time RT-PCR (qRT-PCR) data are normalized with appropriate reference genes. The current exponential increase in postgenomic ...
Selection of reference genes for expression analyses in liver of rats with impaired glucose metabolism
(E-CENTURY PUBLISHING CORP, 2015)
HOXA cluster gene expression during osteoblast differentiation involves epigenetic control
(2019-08-01)
The HOXA gene cluster is generally recognized as a pivotal mediator of positional identity in the skeletal system, expression of different orthologues conferring alternative locational phenotype of the vertebrate bone. ...
Identification of suitable reference genes by quantitative real-time PCR for gene expression normalization in sunflower
(Southern Cross Publishing, 2017-07)
Quantitative real-time PCR (qPCR) is currently the most accurate method for detecting differential gene expression, but depends greatly on normalization to stably expressed housekeeping genes. Transcriptomics analyses and ...