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The multigene families of actinoporins (part II): Strategies for heterologous production in Escherichia coli
(Pergamon-Elsevier Science Ltd, 2016-08)
The sea anemone venom contains pore-forming proteins (PFP) named actinoporins, due to their purification from organisms belonging to Actiniaria order and its ability to form pores in sphingomyelin-containing membranes. ...
Cloning, purification and characterization of nigrelysin, a novel actinoporin from the sea anemone Anthopleura nigrescens
(2019)
Actinoporins constitute a unique class of pore-forming toxins found in sea anemones that being secreted
as soluble monomers are able to bind and permeabilize membranes leading to cell death. The interest in
these proteins ...
The Isolation of new pore-forming toxins from the sea anemone actinia fragacea provides insights into the mechanisms of actinoporin evolution
(Multidisciplinary Digital Publishing Institute, 2019-07)
Random mutations and selective pressure drive protein adaptation to the changing demands of the environment. As a consequence, nature favors the evolution of protein diversity. A group of proteins subject to exceptional ...
Differential Effect of Solution Conditions on the Conformation of the Actinoporins Sticholysin II and Equinatoxin II
(Academia Brasileira de Ciências, 2014-12-01)
Actinoporins are a family of pore-forming proteins with hemolytic activity. The structural basis for such activity appears to depend on their correct folding. Such folding encompasses a phosphocholine binding site, a ...
Differential Effect of Solution Conditions on the Conformation of the Actinoporins Sticholysin II and Equinatoxin II
(Academia Brasileira de Ciências, 2015)
Differential Effect of Solution Conditions on the Conformation of the Actinoporins Sticholysin II and Equinatoxin II
(Academia Brasileira de Ciências, 2015)
Purification, cloning and characterization of fragaceatoxin C, a novel actinoporin from the sea anemone Actinia fragacea
(Pergamon-Elsevier Science Ltd, 2009-11)
Actinia fragacea is commonly called the "strawberry" anemone because of the distinctive yellow or green spots displayed on its red column. Its venom contains several haemolytic proteins with a molecular mass of ∼20 kDa ...
Sticholysin I–membrane interaction: An interplay between the presence of sphingomyelin and membrane fluidity
(Elsevier Science, 2014-01)
Sticholysin I (St I) is a pore-forming toxin (PFT) produced by the Caribbean Sea anemone Stichodactyla helianthus belonging to the actinoporin protein family, a unique class of eukaryotic PFT exclusively found in sea ...
Identification of a membrane-bound prepore species clarifies the lytic mechanism of actinoporins
(American Society for Biochemistry and Molecular Biology, 2016-09)
Pore-forming toxins (PFTs) are cytolytic proteins belonging to the molecular warfare apparatus of living organisms. The assembly of the functional transmembrane pore requires several intermediate steps ranging from a ...
Validation of a mutant of the pore-forming toxin sticholysin-I for the construction of proteinase-activated immunotoxins
(Oxford University Press, 2011-06-01)
The use of pore-forming toxins from sea anemones (actinoporins) in the construction of immunotoxins (ITs) against tumour cells is an alternative for cancer therapy. However, the main disadvantage of actinoporin-based ITs ...