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RNA extraction from the yeast candida parapsilosis sensu stricto using two commercial methods based on purification by silica columns
(2018)
Good quality RNA needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and biological study species. ...
Expression, purification, and circular dichroism analysis of human CDK9
(Elsevier B.V., 2006-06-01)
The human cyclin-dependent kinase 9 (CDK9) protein was expressed in E coli BL21 using the pET23a vector at 30 degrees C. Several milligrams of protein were purified from soluble fraction using ionic exchange and ATP-affinity ...
Expression, purification, and circular dichroism analysis of human CDK9
(Elsevier B.V., 2006-06-01)
The human cyclin-dependent kinase 9 (CDK9) protein was expressed in E coli BL21 using the pET23a vector at 30 degrees C. Several milligrams of protein were purified from soluble fraction using ionic exchange and ATP-affinity ...
High-throughput cloning, expression and purification of glycoside hydrolases using Ligation-Independent Cloning (LIC)
(ElsevierAmsterdam, 2014-07)
Recent advances in DNA sequencing techniques have led to an explosion in the amount of available genome sequencing data and this provided an inexhaustible source of uncharacterized glycoside hydrolases (GH) to be studied ...
Expression and purification of a recombinant adenovirus fiber knob in a baculovirus system
(KARGER, 2008)
Objectives: To construct a recombinant baculovirus expressing the fiber knob domain of human adenovirus type 2 modified by the insertion of a foreign peptide, purify this protein after its production in insect cells, and ...
Synergy of DNA-bending nucleoid proteins and macromolecular crowding in condensing DNA
(2007-10-01)
Many prokaryotic nucleoid proteins bend DNA and form extended helical protein-DNA fibers rather than condensed structures. On the other hand, it is known that such proteins (such as bacterial HU) strongly promote DNA ...
Synergy of DNA-bending nucleoid proteins and macromolecular crowding in condensing DNA
(2007-10-01)
Many prokaryotic nucleoid proteins bend DNA and form extended helical protein-DNA fibers rather than condensed structures. On the other hand, it is known that such proteins (such as bacterial HU) strongly promote DNA ...