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Effect of Polyethylene Glycol-Induced Molecular Crowding on the Enzymatic Activity and Thermal Stability of β-Galactosidase from Kluyveromyces lactis
(American Chemical Society, 2020-08)
Here, we report the effect of polyethylene glycol (PEG6000)-induced molecular crowding (MC) on the catalytic activity and thermal stability of Kluyveromyces lactis β-galactosidase (β-Gal). The β-Gal-catalyzed hydrolysis ...
Interação entre a enzima recombinante agalsidade beta utilizada no tratamento dos pacientes com a doença de Fabry e a atividade da enzima conversora de agiotensina I (ECA I)
(Universidade Federal de São Paulo (UNIFESP), 2011)
Convenient synthesis of 4-thiolactose, 3,4-dithiolactose and related thiooligosaccharides and disulfides. Inhibitory activity of the glycomimetics against a β-galactosidase
(Royal Society of Chemistry, 2012-11)
The ring-opening reaction of sugar 3,4-epoxides by 2,3,4,6-tetra-O-acetyl- 1-thio-β-d-galactopyranose (7) as a nucleophile led to (1 → 3)- and (1 → 4)-thiodisaccharides. High regio- and diastereoselectivities were achieved ...
Response surface methodology as a tool for modelling galacto-oligosaccharide production
(Cambridge University Press, 2017-11)
The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time. The ...
Galacto-oligosaccharides formation during manufacture of different varieties of yogurt. Stability through storage
(Elsevier Science, 2015-09)
Galacto-oligosaccharides (GOS) have interest in the food industry due to their recognized functional properties. In this work, we studied the effect of a commercial β-galactosidase enzyme from Kluyveromyces lactis (YNL-2, ...