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Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis
(American Society for Microbiology, 2011-05)
We tested a new method for detecting drug-resistant strains of Mycobacterium tuberculosis that uses a TM4 mycobacteriophage phAE87::hsp60-EGFP (EGFP-phage) engineered to contain the gene encoding enhanced green fluorescent ...
Neuronal differentiation in the adult hippocampus recapitulates embryonic development
(Society for Neuroscience, 2005-11)
In the adult hippocampus and olfactory bulb, neural progenitor cells generate neurons that functionally integrate into the existing circuits. To understand how neuronal differentiation occurs in the adult hippocampus, we ...
Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus
(Nature Publishing Group, 2001-05)
The administration of leptin to leptin-deficient humans, and the analogous Lepob/Lepob mice, effectively reduces hyperphagia and obesity. But common obesity is associated with elevated leptin, which suggests that obese ...
Expression of exogenous genes in Trypanosoma cruzi: Improving vectors and electroporation protocols
(Springer, 2004-02)
To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol and expression vectors which use luciferase and green and red fluorescent proteins as reporter genes. In transient ...
Long-lasting training in the Barnes maze prompts hippocampal spinogenesis and habituation in rats
(Lippincott Williams, 2017-04)
There is a constant need to assess spatial memory in small rodents to elucidate the basics of cognition in neuroscience experiments. Thus, the significance of the Barnes maze in the biology of hippocampal and cortical ...
Lentivector Transduction Improves Outcomes Over Transplantation of Human HSCs Alone in NOD/SCID/Fabry Mice
(Nature Publishing Group, 2012-07)
Fabry disease is a lysosomal storage disorder caused by a deficiency of a-galactosidase A (a-gal A) activity that results in progressive globotriaosylceramide (Gb(3)) deposition. We created a fully congenic nonobese diabetic ...
Carbon cycling in Anabaena sp. PCC 7120. Sucrose synthesis in the heterocysts and possible role in nitrogen fixation
(American Society of Plant Biologist, 2007-03)
Nitrogen (N) available to plants mostly originates from N2 fixation carried out by prokaryotes. Certain cyanobacterial species contribute to this energetically expensive process related to carbon (C) metabolism. Several ...
An Experimental and Theoretical study of the Photoisomerizacion and Thermal Reversion of 5-arylmethylene-2-thioxoimidazolidin-4-one
(Royal Society of Chemistry, 2015-04-08)
Unraveling the photochemical behaviour of the green fluorescent protein chromophore has lately attracted widespread attention among scientists. In this paper we present the study of the photochemical isomerization Z → E ...
Avaliação da atividade promotora dos genes codificadores de fatores sigma em Corynebacterium pseudotuberculosis em resposta a estresses abióticos
(Universidade Federal de Minas GeraisBrasilICB - DEPARTAMENTO DE BIOLOGIA GERALPrograma de Pós-Graduação em GenéticaUFMG, 2018-05-30)
Corynebacterium pseudotuberculosis (Cp) is a Gram-positive bacterium and the etiological agent of Caseous Lymphadenitis, a disease that causes great economic losses in the sheep and goat industries worldwide. This pathogen ...
Efeitos da ativina A, inibina A e folistatina Sobre células endometriais em um modelo de adesão e invasão peritoneal in vitro.
(Universidade Federal de Minas GeraisUFMG, 2007-10-11)
Objective: The aim of this study was to investigate whether activin A has an effect in the attachment and invasion of endometrial cells in a modeled peritoneum in vitro.Methods: Cultured endometrial stromal and epithelial ...