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Interaction between trypsin and alginate: An ITC and DLS approach to the formation of insoluble complexes
(Elsevier, 2017)
Trypsin is a protease widely used in several industrial areas for leather and meat softening and to produce enzymatic detergents, among others applications. The high demand for this enzyme has motivated the development of ...
Analysis of toxoplasma gondii proteins after Triton X-114 solubilization and hidropholic chromotograhy
(Fundação Oswaldo Cruz. Instituto Oswaldo Cruz., 2020)
Identification, characterization, and purification of xylanase PC-01 isolated from Pacet hot spring
(Universidad del Zulia, 2019)
Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography
(SPRINGER, 2008-08)
During cationic bed adsorption (EBA), with cutinase with varying length tryptophan tags (WP)(2) and (WP)(4), 33% and 10% of adsorption capacity and 80% and 32% eluted specific activity were observed in relation to wild ...
Prediction of protein retention in hydrophobic interaction chromatography
(PERGAMON-ELSEVIER SCIENCE, 2005-07)
Hydrophobic interaction chromatography (HIC) is a powerful technique for protein separation. This review examines methodologies for predicting protein retention time in HIC involving elution with salt gradients. The ...
Hydrophobic interaction chromatography for purification of monoPEGylated RNase A
(ELSEVIER SCIENCE BV, 2012-06-15)
The chromatographic methods used for the purification of PEGylated proteins are mainly Size Exclusion
(SEC) and Ion Exchange Chromatography (IEX). Although the PEGylation affects the protein hydrophobicity,
Hydrophobic ...