Artículo de revista
Prediction of protein retention in hydrophobic interaction chromatography
Fecha
2005-07Registro en:
BIOTECHNOLOGY ADVANCES 23 (5): 359-368 JUL 2005
0734-9750
Autor
Mahn Osses, Andrea
Asenjo de Leuze, Juan
Institución
Resumen
Hydrophobic interaction chromatography (HIC) is a powerful technique for protein separation. This review examines methodologies for predicting protein retention time in HIC involving elution with salt gradients. The methodologies discussed consider three-dimensional structure data of the protein and its surface hydrophobicity. Despite their limitations, the methods discussed are useful in designing purification processes for proteins and easing the tedious experimental work that is currently required for developing purification protocols.