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Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions
(Biomed Central Ltd., 2009-01-06)
Background: Quantitative data from gene expression experiments are often normalized by transcription levels of reference or housekeeping genes. An inherent assumption for their use is that the expression of these genes is ...
A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression
(Amer Soc Clinical Investigation Inc, 2004-04-01)
Accurate diagnosis of thyroid tumors is challenging. A particular problem is distinguishing between follicular thyroid carcinoma (FTC) and benign follicular thyroid adenoma (FTA), where histology of fine-needle aspirates ...
Suppression of Myc oncogenic activity by ribosomal protein haploinsufficiency
(NATURE PUBLISHING GROUP, 2008)
The Myc oncogene regulates the expression of several components of the protein synthetic machinery, including ribosomal proteins, initiation factors of translation, RNA polymerase III and ribosomal DNA(1,2). Whether and ...
Selection and optimization of reference genes for RT-qPCR normalization: a case study in Solanum lycopersicum exposed to UV-B
(Elsevier France-Editions Scientifiques Medicales Elsevier, 2021-03-22)
Quantitative RT- PCR is one of the most common methods to study gene expression in response to stress. Therefore, it is crucial to have suitable reference genes (RGs) for result normalization. Although several reports ...