Artículos de revistas
Response Surface Analysis For The Production Of An Enantioselective Lipase From Aspergillus Niger By Solid-state Fermentation.
Registro en:
Journal Of Microbiology (seoul, Korea). v. 47, n. 5, p. 563-71, 2009-Oct.
1976-3794
10.1007/s12275-008-0279-8
19851729
Autor
Contesini, Fabiano Jares
da Silva, Vania Castriani Fernades
Maciel, Rafael Ferreira
de Lima, Rosemary Joana
Barros, Francisco Fábio Cavalcante
de Oliveira Carvalho, Patrícia
Institución
Resumen
The lipase produced by the Aspergillus niger strain AC-54 has been widely studied due to its enantioselectivity for racemic mixtures. This study aimed to optimize the production of this enzyme using statistical methodology. Initially a Plackett-Burman (PB) design was used to evaluate the effects of the culture medium components and the culture conditions. Twelve factors were screened: water content, glucose, yeast extract, peptone, olive oil, temperature, NaH(2)P0(4), KH(2)P0(4), MgS0(4)-7H(2)0, CaCl(2), NaCI, and MnS0(4). The screening showed that the significant factors were water content, glucose, yeast extract, peptone, NaH(2)P0(4), and KH(2)P0(4), which were optimized using response surface methodology (RSM) and a mathematical model obtained to explain the behavioral process. The best lipase activity was attained using the following conditions: water content (20%), glucose (4.8%), yeast extract (4.0%), and NaH2P04 (4.0%). The predicted lipase activity was 33.03 U/ml and the experimental data confirmed the validity of the model. The enzymatic activity was expressed as micromoles of oleic acid released per minute of reaction (micromol/min). 47 563-71