dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorStasevich, Timothy J.
dc.date2016-10-26T17:56:52Z
dc.date2016-10-26T17:56:52Z
dc.date.accessioned2017-04-06T12:14:25Z
dc.date.available2017-04-06T12:14:25Z
dc.identifierhttp://acervodigital.unesp.br/handle/unesp/365212
dc.identifierhttp://objetoseducacionais2.mec.gov.br/handle/mec/16128
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/961494
dc.descriptionFluorescence recovery after photobleaching (FRAP) can be used to quantify the mobility of molecules in live cells. In a typical FRAP experiment, a portion of a cell expressing a fluorescently tagged molecule of interest is photobleached and the recovery of the photobleached region is monitored as a function of time. Briefly, faster recoveries correspond to more mobile molecules. In this Demonstration a movie of a FRAP experiment is analyzed. You can scroll through the movie in the upper display and choose the size and position of a circular region of interest (ROI). The normalized intensity of the fluorescence in the ROI is displayed as a function of image number in a plot below. A vertical red line designates the current image number of the movie in the upper display
dc.descriptionComponente Curricular::Educação Superior::Multidisciplinar::Interdiciplinar
dc.publisherWolfram Demonstration Project
dc.relationASelectionAndAnalysisToolForFluorescenceRecoveryAfterPhotobl.nbp
dc.rightsDemonstration freeware using MathematicaPlayer
dc.subjectData analysis
dc.subjectEducação Superior::Multidisciplinar::Ensino::Ensino de Ciências e Matemática
dc.titleA selection and analysis tool for fluorescence recovery after photobleaching (FRAP) region of interest (ROI)
dc.typeOtro


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