dc.contributor | Universidade Estadual Paulista (UNESP) | |
dc.creator | Stasevich, Timothy J. | |
dc.date | 2016-10-26T17:56:52Z | |
dc.date | 2016-10-26T17:56:52Z | |
dc.date.accessioned | 2017-04-06T12:14:25Z | |
dc.date.available | 2017-04-06T12:14:25Z | |
dc.identifier | http://acervodigital.unesp.br/handle/unesp/365212 | |
dc.identifier | http://objetoseducacionais2.mec.gov.br/handle/mec/16128 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/961494 | |
dc.description | Fluorescence recovery after photobleaching (FRAP) can be used to quantify the mobility of molecules in live cells. In a typical FRAP experiment, a portion of a cell expressing a fluorescently tagged molecule of interest is photobleached and the recovery of the photobleached region is monitored as a function of time. Briefly, faster recoveries correspond to more mobile molecules. In this Demonstration a movie of a FRAP experiment is analyzed. You can scroll through the movie in the upper display and choose the size and position of a circular region of interest (ROI). The normalized intensity of the fluorescence in the ROI is displayed as a function of image number in a plot below. A vertical red line designates the current image number of the movie in the upper display | |
dc.description | Componente Curricular::Educação Superior::Multidisciplinar::Interdiciplinar | |
dc.publisher | Wolfram Demonstration Project | |
dc.relation | ASelectionAndAnalysisToolForFluorescenceRecoveryAfterPhotobl.nbp | |
dc.rights | Demonstration freeware using MathematicaPlayer | |
dc.subject | Data analysis | |
dc.subject | Educação Superior::Multidisciplinar::Ensino::Ensino de Ciências e Matemática | |
dc.title | A selection and analysis tool for fluorescence recovery after photobleaching (FRAP) region of interest (ROI) | |
dc.type | Otro | |