Presents mitotic PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respectively. The movie starts in metaphase and shows sister kinetochore pairs aligned at the metaphase and oscillating between the two spindle poles as their respective kinetochore fibers lengthen and shorten. As the cell enters anaphase and the sister chromatids separate, sister kinetochores move away from each other as their kinetochore fibers shorten (anaphase A). Almost simultaneously, the two spindle poles move away from each other leading to elongation of the mitotic spindle (anaphase B). The compacted DNA of the chromosome arms excludes the background fluorescence so that chromosome arms appear as dark rod-like structures and the movement of the sister chromatid arms away from each other during anaphase can also be followedComponente Curricular::Educação Superior::Ciências Biológicas::Biologia Geral