Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells

dc.creatorCorrêa De Melo, Thatiana
dc.creatorTrevisan Silva, Dilza
dc.creatorAlvarez Flores, Miryam P.
dc.creatorNascimento Gomes, Renata
dc.creatorMedina De Souza, Marcelo
dc.creatorValerio, Hellen Paula
dc.creatorOliveira, Douglas S.
dc.creatorDeOcesano Pereira, Carlos
dc.creatorFongaro Botosso, Viviane
dc.creatorCalil Jorge, Soraia Attie
dc.creatorSchattner, Mirta
dc.creatorGómez, Ricardo Martín
dc.creatorChudzinski Tavassi, Ana Marisa
dc.date2022-09
dc.date2023-08-28T18:27:32Z
dc.date.accessioned2024-07-24T03:46:51Z
dc.date.available2024-07-24T03:46:51Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/156970
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9535158
dc.descriptionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanced immune response. The molecular mechanisms underlying endothelial activation have not been fully elucidated. We aimed to identify the proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, which might help to unravel the molecular mechanisms of endothelium activation in COVID-19. In this direction, we exposed HUVECs to SARS-CoV-2 and analyzed the expression of specific cellular receptors, and changes in the proteome of HUVECs at different time points. We identified that HUVECs exhibit non-productive infection without cytopathic effects, in addition to the lack of expression of specific cell receptors known to be essential for SARS-CoV-2 entry into cells. We highlighted the enrichment of the protein SUMOylation pathway and the increase in SUMO2, which was confirmed by orthogonal assays. In conclusion, proteomic analysis revealed that the exposure to SARS-CoV-2 induced oxidative stress and changes in protein abundance and pathways enrichment that resembled endothelial dysfunction.
dc.descriptionInstituto de Biotecnología y Biología Molecular
dc.formatapplication/pdf
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectCiencias Exactas
dc.subjectBiología
dc.subjectSARS-CoV-2
dc.subjectendothelial cells
dc.subjectproteomics
dc.subjectmass spectrometry
dc.subjectHUVECs
dc.titleProteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
dc.typeArticulo
dc.typeArticulo


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