Argentina | Articulo

Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry

dc.creatorRimondi, Agustina
dc.creatorOlivera, Valeria S.
dc.creatorSoria, Ivana
dc.creatorParisi, Gustavo Daniel
dc.creatorRumbo, Martín
dc.creatorPérez, Daniel R.
dc.date2022
dc.date2023-08-04T17:14:38Z
dc.date.accessioned2024-07-24T03:26:23Z
dc.date.available2024-07-24T03:26:23Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/155926
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9534157
dc.descriptionIn chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3CTCRgdC IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.
dc.descriptionInstituto de Estudios Inmunológicos y Fisiopatológicos
dc.formatapplication/pdf
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectBiología
dc.subjectCiencias Veterinarias
dc.subjectinfluenza A virus
dc.subjectSouth American IAV
dc.subjectviral adaptation
dc.subjectviral fitness
dc.subjectviral transmission
dc.subjectchicken studies
dc.subjectrisk to poultry
dc.subjectimmunopathogenesis
dc.titleFew amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
dc.typeArticulo
dc.typeArticulo


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