The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPedraza-Ordoñez, Francisco
dc.creatorYamatogi, Ricardo Seidi
dc.creatorAraújo Júnior, João Pessoa
dc.creatorRocha, Noeme Souza
dc.creatorAlessi, Antonio Carlos
dc.date2016-07-07T12:36:46Z
dc.date2016-10-25T21:45:18Z
dc.date2016-07-07T12:36:46Z
dc.date2016-10-25T21:45:18Z
dc.date2013
dc.date.accessioned2017-04-06T10:49:28Z
dc.date.available2017-04-06T10:49:28Z
dc.identifierJournal of Veterinary Science & Technology, v. 4, n. 5, p. 1-5, 2013.
dc.identifier2157-7579
dc.identifierhttp://hdl.handle.net/11449/141083
dc.identifierhttp://acervodigital.unesp.br/handle/11449/141083
dc.identifier10.4172/2157-7579.1000145
dc.identifierISSN2157-7579-2013-04-05-01-05.pdf
dc.identifier3740074012748150
dc.identifierhttp://dx.doi.org/10.4172/2157-7579.1000145
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/951367
dc.descriptionBackground: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas. Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if amplification occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise. Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis.
dc.languageeng
dc.relationJournal of Veterinary Science & Technology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBovine herpes virus 5
dc.subjectReal-time PCR
dc.subjectDiagnosis
dc.subjectBoHV-5
dc.titleThe use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis
dc.typeOtro


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