Improvement in fractional shortening in aortic regurgitation rats: cardiac muscle network

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOmoko, Ana Carolina Mieko
dc.creatorFernandez, Geysson
dc.creatorMoraes, Leonardo Nazario
dc.creatorRoscani, Meliza Goi
dc.creatorCarvalho, Robson Francisco
dc.creatorGobbi, Juliana Irani Fratucci de
dc.date2016-07-07T12:35:38Z
dc.date2016-10-25T21:44:40Z
dc.date2016-07-07T12:35:38Z
dc.date2016-10-25T21:44:40Z
dc.date2015
dc.date.accessioned2017-04-06T10:47:25Z
dc.date.available2017-04-06T10:47:25Z
dc.identifierThe FASEB Journal, v. 29, p. 799.6, 2015.
dc.identifier1530-6860
dc.identifierhttp://hdl.handle.net/11449/140835
dc.identifierhttp://acervodigital.unesp.br/handle/11449/140835
dc.identifier0000-0002-4901-7714
dc.identifier1298051150234140
dc.identifierhttp://www.fasebj.org/content/29/1_Supplement/799.6
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/951121
dc.descriptionAortic regurgitation (AR), a volume overload to the heart, impairs systolic function. Paroxetine (parox) treatment, a selective serotonin reuptake inhibitor, improves systolic function in AR rats, probably due decreases in the expression of β-myosin heavy chain (βMyHC) gene. An intricate network regulate genes co-expressing microRNAs (miR-208a,-208b and -499) and transcriptional repressors which in turn controls MyHCisoforms content. Thus, we verify the gene expression of those microRNAs and the transcriptional repressor (Thrap1) in AR rats treated with parox. Male Wistar rats (280-300kg) were submitted to sham or AR surgery. Morphofunctional variables of the hearts were analyzed by echocardiograms. Parox (10mg/kg) was administered subcutaneously for 4 weeks. There were 4 groups: AR+parox, AR+saline, Sham+parox and Sham+saline. At week 8 the animals were euthanized for tissue collection and analysis of gene expression by RTq-PCR. Two way repeated measures ANOVA were used for comparisons. Parox treatment preserved the fractional shortening in AR rats. The expression of miR-208a and Thrap 1 were not changed. Though there was a decrease in miR-208b and miR-499 gene expression, which may regulate the decrease of β-MyHC isoform in AR rats treated with parox, explaining the improvement in systolic function.
dc.descriptionFundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationThe FASEB Journal
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleImprovement in fractional shortening in aortic regurgitation rats: cardiac muscle network
dc.typeOtro


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