Expression of growth-related factors in skeletal muscle of Pirarucu (Arapaima gigas) during growth

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCarani, Fernanda Regina
dc.creatorDuran, Bruno Oliveira da Silva
dc.creatorPiedade, Warlen Pereira
dc.creatorCosta, Fernanda Antunes Alves da
dc.creatorVal, Vera Maria Fonseca de Almeida
dc.creatorSilva, Maeli Dal Pai
dc.date2016-04-01T18:44:50Z
dc.date2016-10-25T21:36:53Z
dc.date2016-04-01T18:44:50Z
dc.date2016-10-25T21:36:53Z
dc.date2014
dc.date.accessioned2017-04-06T10:19:34Z
dc.date.available2017-04-06T10:19:34Z
dc.identifierJournal of Aquaculture Research and Development, v. 5, n. 6, p. 1-7, 2014.
dc.identifier2155-9546
dc.identifierhttp://hdl.handle.net/11449/137237
dc.identifierhttp://acervodigital.unesp.br/handle/11449/137237
dc.identifier10.4172/2155-9546.1000272
dc.identifierISSN2155-9546-2014-05-06-01-07.pdf
dc.identifier4502916373369334
dc.identifier2573718989102992
dc.identifierhttp://dx.doi.org/10.4172/2155-9546.1000272
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/947758
dc.descriptionThe skeletal muscle of the species Arapaima gigas (pirarucu) constitutes the major edible part of the fish and is, therefore, an important protein source for human consumption. Post-natal muscle growth is regulated by the expression of myostatin as well as the Myogenic Regulatory Factors (MRFs) MyoD and myogenin. Once pirarucu reaches large size, we assume that the control of muscle growth by MRFs and myostatin occurs differentially at the initials life stages. In the present work we evaluated the morphological aspects and expression of MRF genes in skeletal muscle of pirarucu during early juvenile stage (Group A, up to 50 g, n=7), and post juvenile stages (Groups B, from 50 to 400 g, n=7; C, from 400 g to 5 kg, n=7, and D, from 5 to 9 kg, n=7). Transverse sections of red and white muscles were obtained to evaluate muscle fiber morphological and morphometric characteristics. MyoD, myogenin and myostatin genes and protein expressions were determined after quantitative real-time polymerase chain reaction and western blotting, respectively. Pirarucu skeletal muscle exhibited similar morphologies at different life stages. It was possible to conclude that both hyperplasia and hypertrophy occur during muscle growth in early and postjuvenile stages. As regard as expression, both mRNA and protein levels were similar among all groups for MyoD and myogenin. Myostatin presented lower mRNA levels and higher protein levels in early-juvenile stage, compared with the other groups. The levels of MRFs and myostatin might be involved in a balance that controls hyperplasia and hypertrophy occurring during post-natal muscle growth. Myostatin does not appear to play a crucial role during pirarucu early-juvenile stages. Since this species represents an interesting model for aquaculture programs due to high growing rates, our data suggest that the best phase to improve muscle growth in pirarucu is at post-juvenile stage and this will turn the employment of pirarucu farming economically better.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.relationJournal of Aquaculture Research and Development
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFish skeletal muscle
dc.subjectMuscle growth
dc.subject46 Myogenic regulatory factors
dc.subjectMyostatin
dc.subjectRT-qPCR
dc.subjectWestern blotting
dc.subjectPirarucu arapaima gigas
dc.titleExpression of growth-related factors in skeletal muscle of Pirarucu (Arapaima gigas) during growth
dc.typeOtro


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