Inhibitory effect of gallic acid and its esters on 2,2'-azobis(2-amidinopropane)hodrpchloride (AAPH)-induced hemolysis and depletion of intracellular glutathione in erythrocytes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorXimenes, Valdecir Farias
dc.creatorLopes, Mariana G.
dc.creatorPetrônio, Maicon Segalla
dc.creatorRegasini, Luis Octavio
dc.creatorSilva, Dulce Helena Siqueira
dc.creatorFonseca, Luiz Marcos da
dc.date2016-01-28T16:56:18Z
dc.date2016-10-25T21:28:43Z
dc.date2016-01-28T16:56:18Z
dc.date2016-10-25T21:28:43Z
dc.date2010
dc.date.accessioned2017-04-06T09:49:54Z
dc.date.available2017-04-06T09:49:54Z
dc.identifierJournal of Agricultural and Food Chemistry, v. 58, p. 5355-5362, 2010.
dc.identifier0021-8561
dc.identifierhttp://hdl.handle.net/11449/133717
dc.identifierhttp://acervodigital.unesp.br/handle/11449/133717
dc.identifier10.1021/jf100233y
dc.identifier4419635633356792
dc.identifier4066413997908572
dc.identifier0992736452764550
dc.identifier4702004904231248
dc.identifierhttp://pubs.acs.org/doi/abs/10.1021/jf100233y
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/944247
dc.descriptionThe protective effect of gallic acid and its esters, methyl, propyl, and lauryl gallate, against 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH)-induced hemolysis and depletion of intracellular glutathione (GSH) in erythrocytes was studied. The inhibition of hemolysis was dose-dependent, and the esters were significantly more effective than gallic acid. Gallic acid and its esters were compared with regard to their reactivity to free radicals, using the DPPH and AAPH/pyranine free-cell assays, and no significant difference was obtained. Gallic acid and its esters not only failed to inhibit the depletion of intracellular GSH in erythrocytes induced by AAPH but exacerbated it. Similarly, the oxidation of GSH by AAPH or horseradish peroxidase/H(2)O(2) in cell-free systems was exacerbated by gallic acid or gallates. This property could be involved in the recent findings on pro-apoptotic and pro-oxidant activities of gallates in tumor cells. We provide evidence that lipophilicity and not only radical scavenger potency is an important factor regarding the efficiency of antihemolytic substances.
dc.languageeng
dc.relationJournal of Agricultural and Food Chemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGallic acid
dc.subjectGallates
dc.subjectErythrocytes
dc.subjectHemolysis
dc.subjectGlutathione
dc.subjectPeroxyl radical
dc.subjectAntioxidant
dc.subjectPro-oxidant activity
dc.titleInhibitory effect of gallic acid and its esters on 2,2'-azobis(2-amidinopropane)hodrpchloride (AAPH)-induced hemolysis and depletion of intracellular glutathione in erythrocytes
dc.typeOtro


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