Raloxifene modulates regulators of osteoclastogenesis and angiogenesis in an oestrogen deficiency periapical lesion model

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGomes Filho, João Eduardo
dc.creatorWayama, Marcelo Tadahiro
dc.creatorDornelles, R. C. M.
dc.creatorErvolino, Edilson
dc.creatorYamanari, Guilherme Hiroshi
dc.creatorLodi, C. S.
dc.creatorSivieri-Araújo, G.
dc.creatorDezan Júnior, Eloi
dc.creatorCintra, Luciano Tavares Angelo
dc.date2016-01-28T16:55:24Z
dc.date2016-10-25T21:28:14Z
dc.date2016-01-28T16:55:24Z
dc.date2016-10-25T21:28:14Z
dc.date2014
dc.date.accessioned2017-04-06T09:48:10Z
dc.date.available2017-04-06T09:48:10Z
dc.identifierInternational Endodontic Journal, v. 48, n. 11, p. 1059-1068, 2014.
dc.identifier0143-2885
dc.identifierhttp://hdl.handle.net/11449/133508
dc.identifierhttp://acervodigital.unesp.br/handle/11449/133508
dc.identifier10.1111/iej.12403
dc.identifier8813777272581972
dc.identifier5192720917324352
dc.identifier9235743081667362
dc.identifierhttp://dx.doi.org/10.1111/iej.12403
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/944038
dc.descriptionAim To analyse the local regulatory mechanisms of osteoclastogenesis and angiogenesis during the progression of periapical lesions in female rats with oestrogen deficiency and treatment with raloxifene (RLX). Methodology Female Wistar rats were distributed into groups: SHAM-veh, subjected to sham surgery and treated with a vehicle; OVX-veh, subjected to ovary removal and treated with a vehicle; and OVX-RLX, subjected to ovary removal and treated with RLX. Vehicle or RLX was administered orally for 90 days. During treatment, the dental pulp of mandibular first molars was exposed to the oral environment for induction of periapical lesions, which were analysed after 7 and 30 days. After the experimental periods, blood samples were collected for measurement of oestradiol, calcium, phosphorus and alkaline phosphatase. The rats were euthanized and the mandibles removed and processed for immunohistochemical detection of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), hypoxia-inducible factor-1 alpha (HIF-1α) and bone-specific alkaline phosphatase (BALP). Data were compared using Kruskal–Wallis followed by Dunn test (nonparametric values) and anova followed by the Tukey's test (parametric values). Results The plasma concentration of oestradiol showed hypo-oestrogenism in the rats subjected to ovary removal. On day 7, alkaline phosphatase activity, calcium and phosphorus were higher in the OVX-RLX group than in the OVX-veh group (P < 0.001), but immunolabelling for RANKL and HIF-1α was lower in OVX-RLX group (P < 0.001). On day 30, the OVX-veh group had higher immunolabelling for RANKL than the OVX-RLX group (P < 0.05). There were no significant differences in the immunoreactivity of OPG and BALP between any groups at either time-point (P > 0.05). Conclusion RLX therapy reversed the increased levels of the local regulators of both osteoclastogenesis and angiogenesis induced by oestrogen deficiency.
dc.languageeng
dc.relationInternational Endodontic Journal
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBone metabolism
dc.subjectOvariectomized
dc.subjectPeriapical lesion
dc.subjectRaloxifene hydrochloride
dc.titleRaloxifene modulates regulators of osteoclastogenesis and angiogenesis in an oestrogen deficiency periapical lesion model
dc.typeOtro


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