Pathology and virus tissue distribution of Turkey Coronavirus (TCoV) in experimentally infected chicks and turkey poults

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGomes, Deriane Elias
dc.creatorHirata, Karina Yukie
dc.creatorSaheki, Karina
dc.creatorRosa, Ana Carolina Guedes
dc.creatorLuvizotto, Maria Cecília Rui
dc.creatorCardoso, Tereza Cristina
dc.date2016-01-28T16:53:41Z
dc.date2016-10-25T21:27:18Z
dc.date2016-01-28T16:53:41Z
dc.date2016-10-25T21:27:18Z
dc.date2010
dc.date.accessioned2017-04-06T09:44:58Z
dc.date.available2017-04-06T09:44:58Z
dc.identifierJournal of Comparative Pathology, v. 23, n. 1, p. 8-13, 2010.
dc.identifier0021-9975
dc.identifierhttp://hdl.handle.net/11449/133113
dc.identifierhttp://acervodigital.unesp.br/handle/11449/133113
dc.identifier10.1016/j.jcpa.2009.12.012
dc.identifier4584674909952477
dc.identifier6069686231701579
dc.identifier4026608288401807
dc.identifier5064990640039641
dc.identifier4272904699684196
dc.identifierhttp://dx.doi.org/10.1016/j.jcpa.2009.12.012
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/943644
dc.descriptionTwenty 1-day-old specific pathogen free chicks and 20 1-day-old commercially derived turkey poults were inoculated with a Brazilian strain of turkey coronavirus (TCoV) to study the pathogenicity and virus distribution up to 14 days post-inoculation by histopathology, immunohistochemistry, reverse transcriptase polymerase chain reaction and sequencing. At 2–14 dpi, TCoV antigens were detected in the paranasal sinus and lachrymal accessory gland (Harderian gland) of infected chicks and in the ileum, ileocaecal junction and caecum of infected poults. Lymphocytic inflammation was present in these tissues. TCoV was re-isolated from pooled tissue suspensions of nasal concha, Harderian gland and paranasal sinus from chicks, as well as from the ileum, ileocaecal junction and caecum of poults, after three consecutive passages in 28-day-old embryonated turkey eggs. Viral RNA corresponding to the spike gene region (1178–2073 genome position) was amplified from the upper respiratory tract of chickens and from the intestinal tract of poults and phylogenetic analysis confirmed the identity as TCoV. This is the first description of TCoV antigens and mRNA in upper respiratory tissues in experimentally infected chickens.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.relationJournal of Comparative Pathology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectImmunohistochemistry
dc.subjectPathology
dc.subjectTurkey coronavirus
dc.subjectViral infection
dc.titlePathology and virus tissue distribution of Turkey Coronavirus (TCoV) in experimentally infected chicks and turkey poults
dc.typeOtro


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