Tumour necrosis factor-alpha-induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGarcia, Juliana Albarracin
dc.creatorFerreira, Helena Lage
dc.creatorVieira, Flavia Volpato
dc.creatorGameiro, Roberto
dc.creatorAndrade, Alexandre Lima de
dc.creatorEugenio, Flavia de Rezende
dc.creatorFlores, Eduardo Furtado
dc.creatorCardoso, Tereza Cristina
dc.date2016-01-28T16:53:41Z
dc.date2016-10-25T21:27:18Z
dc.date2016-01-28T16:53:41Z
dc.date2016-10-25T21:27:18Z
dc.date2015
dc.date.accessioned2017-04-06T09:44:57Z
dc.date.available2017-04-06T09:44:57Z
dc.identifierVeterinary and Comparative Oncology, p. 1-9, 2015.
dc.identifier1476-5829
dc.identifierhttp://hdl.handle.net/11449/133112
dc.identifierhttp://acervodigital.unesp.br/handle/11449/133112
dc.identifier10.1111/vco.12168
dc.identifier4272904699684196
dc.identifier5359531229053723
dc.identifier5431798684243196
dc.identifier6404176495306171
dc.identifierhttp://dx.doi.org/10.1111/vco.12168
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/943643
dc.descriptionOncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus (CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine-derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine-derived adenofibrosarcoma cell line were tested regarding to their susceptibility to CDV infection, cell proliferation, apoptosis, mitochondrial membrane potential and expression of tumour necrosis factor-alpha-induced protein 8 (TNFAIP8). CDV replication-induced cytopathic effect, decrease of cell proliferation rates, and >45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDVM gene expression were positively correlated in all cell lines. In addition, mitochondrial membrane depolarization was associated with increase in virus titres (p < 0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV-induced cancer therapy.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.relationVeterinary and Comparative Oncology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCancer markers
dc.subjectCanine and human mammary tumours
dc.subjectCell phenotype
dc.subjectIn vitro culture
dc.titleTumour necrosis factor-alpha-induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus
dc.typeOtro


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