dc.contributor | Universidade Estadual Paulista (UNESP) | |
dc.creator | Nakamoto, Wilson | |
dc.creator | Machado, Paulo Eduardo de Abreu | |
dc.date | 2014-05-27T02:06:13Z | |
dc.date | 2016-10-25T21:25:31Z | |
dc.date | 2014-05-27T02:06:13Z | |
dc.date | 2016-10-25T21:25:31Z | |
dc.date | 1986-12-01 | |
dc.date.accessioned | 2017-04-06T09:38:05Z | |
dc.date.available | 2017-04-06T09:38:05Z | |
dc.identifier | Comparative Biochemistry and Physiology -- Part B: Biochemistry and, v. 84, n. 3, p. 383-386, 1986. | |
dc.identifier | 0305-0491 | |
dc.identifier | http://hdl.handle.net/11449/132303 | |
dc.identifier | http://acervodigital.unesp.br/handle/11449/132303 | |
dc.identifier | 10.1016/0305-0491(86)90094-5 | |
dc.identifier | WOS:A1986D370700024 | |
dc.identifier | 2-s2.0-0022464335 | |
dc.identifier | http://dx.doi.org/10.1016/0305-0491(86)90094-5 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/942843 | |
dc.description | 1. 1. Total hemolysates of Synbranchus marmoratus Bloch, 1795, captured in Vitoriana, district of Botucatu, State of São Paulo, Brazil, were submitted to agar-starch gel electrophoresis on glass slides using 42 mM-Tris 1.7 mM EDTA-6.1 mM borate buffer, pH 8.8, for the gel and 10 mM borate-1.7 mM NaOH buffer, pH 8.6, for the cuvette. 2. 2. Three distinct hemoglobin bands were detected, with Hb I being of the cathodic type. 3. 3. Cellulose acetate electrophoresis in 800 mM Tris-2.1 mM EDTA buffer, pH 8.9, containing 6 M urea and 2.25 mM β-mercaptoethanol indicated the presence of four globin chains denoted α 1, α 2, β and γ. 4. 4. It is suggested that the probable tetrameric constitution of the hemoglobin of Synbranchus marmoratus Bloch, 1795 is Hb I (α 2 2γ 2), Hb II (α 2 1γ 2) and Hb III (α 2 1β 2). © 1986. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Globin | |
dc.subject | Hemoglobin | |
dc.subject | Agar gel electrophoresis | |
dc.subject | Animal | |
dc.subject | Blood | |
dc.subject | Electrophoresis | |
dc.subject | Fish | |
dc.subject | Ion exchange chromatography | |
dc.subject | Animal | |
dc.subject | Chromatography, DEAE-Cellulose | |
dc.subject | Electrophoresis, Agar Gel | |
dc.subject | Electrophoresis, Cellulose Acetate | |
dc.subject | Fishes | |
dc.subject | Globins | |
dc.subject | Hemoglobins | |
dc.title | Characterization of the hemoglobins and globins of Synbranchus marmoratus Bloch, 1795 (Pisces, Synbranchidae) | |
dc.type | Otro | |