dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorNakamoto, Wilson
dc.creatorMachado, Paulo Eduardo de Abreu
dc.date2014-05-27T02:06:13Z
dc.date2016-10-25T21:25:31Z
dc.date2014-05-27T02:06:13Z
dc.date2016-10-25T21:25:31Z
dc.date1986-12-01
dc.date.accessioned2017-04-06T09:38:05Z
dc.date.available2017-04-06T09:38:05Z
dc.identifierComparative Biochemistry and Physiology -- Part B: Biochemistry and, v. 84, n. 3, p. 383-386, 1986.
dc.identifier0305-0491
dc.identifierhttp://hdl.handle.net/11449/132303
dc.identifierhttp://acervodigital.unesp.br/handle/11449/132303
dc.identifier10.1016/0305-0491(86)90094-5
dc.identifierWOS:A1986D370700024
dc.identifier2-s2.0-0022464335
dc.identifierhttp://dx.doi.org/10.1016/0305-0491(86)90094-5
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/942843
dc.description1. 1. Total hemolysates of Synbranchus marmoratus Bloch, 1795, captured in Vitoriana, district of Botucatu, State of São Paulo, Brazil, were submitted to agar-starch gel electrophoresis on glass slides using 42 mM-Tris 1.7 mM EDTA-6.1 mM borate buffer, pH 8.8, for the gel and 10 mM borate-1.7 mM NaOH buffer, pH 8.6, for the cuvette. 2. 2. Three distinct hemoglobin bands were detected, with Hb I being of the cathodic type. 3. 3. Cellulose acetate electrophoresis in 800 mM Tris-2.1 mM EDTA buffer, pH 8.9, containing 6 M urea and 2.25 mM β-mercaptoethanol indicated the presence of four globin chains denoted α 1, α 2, β and γ. 4. 4. It is suggested that the probable tetrameric constitution of the hemoglobin of Synbranchus marmoratus Bloch, 1795 is Hb I (α 2 2γ 2), Hb II (α 2 1γ 2) and Hb III (α 2 1β 2). © 1986.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationComparative Biochemistry and Physiology -- Part B: Biochemistry and
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGlobin
dc.subjectHemoglobin
dc.subjectAgar gel electrophoresis
dc.subjectAnimal
dc.subjectBlood
dc.subjectElectrophoresis
dc.subjectFish
dc.subjectIon exchange chromatography
dc.subjectAnimal
dc.subjectChromatography, DEAE-Cellulose
dc.subjectElectrophoresis, Agar Gel
dc.subjectElectrophoresis, Cellulose Acetate
dc.subjectFishes
dc.subjectGlobins
dc.subjectHemoglobins
dc.titleCharacterization of the hemoglobins and globins of Synbranchus marmoratus Bloch, 1795 (Pisces, Synbranchidae)
dc.typeOtro


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